Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10)
Sample
Mouse Cell lysate - other (C2C12)
Specification
C2C12
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: promega anti-Rabbit Ig HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 115 kDa Non-specific: 80 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
sample
Positive control
brain tissue lysis
Additional data
Additional Notes
Lysis C2C12 cell with cell lysis buffer(include protease inhibitor cocktail ,PMSF, leupeptin, aprotinin,van and so on). After spin down and transfer the supernatant. Finally add 2*SDS buffer and run gel.
We also check the leptin receptor in kidney of mice, the bands were dirty.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 27 2014