Anti-Leptin Receptor 抗体 (ab5593)
Key features and details
- Rabbit polyclonal to Leptin Receptor
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Leptin Receptor antibody
Leptin Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Leptin Receptor -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Leptin Receptor aa 577-594 (extracellular).
Sequence:KEIQWKTHEVFDAKSKSA
(Peptide available asab5837) -
ポジティブ・コントロール
- WB: Mouse lung tissue extract.
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特記事項
As per Uniprot database (P48357) OB receptor has 5 isoforms and is highly glycosylated, this means the observed band size will be different than predicted and multiple bands are expected depending upon the isoforms expressed in sample types.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
特記事項(精製)
No concentration is available. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | 1/2000. Detects a band of approximately 100, 125 kDa.Can be blocked with Leptin Receptor peptide (ab5837). ab5593 detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R.
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ターゲット情報
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機能
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway. -
組織特異性
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus. -
配列類似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain. -
ドメイン
The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻訳後修飾
On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3953 Human
- Entrez Gene: 16847 Mouse
- Entrez Gene: 24536 Rat
- Omim: 601007 Human
- SwissProt: P48357 Human
- SwissProt: P48356 Mouse
- SwissProt: Q62959 Rat
- Unigene: 23581 Human
see all -
別名
- CD 295 antibody
- CD295 antibody
- CD295 antigen antibody
see all
画像
プロトコール
参考文献 (22)
ab5593 は 22 報の論文で使用されています。
- Wu H et al. Blockade of adrenergic ß-receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair in vivo. Cell Prolif 53:e12725 (2020). PubMed: 31746058
- Fischer J et al. Leptin signaling impairs macrophage defenses against Salmonella Typhimurium. Proc Natl Acad Sci U S A 116:16551-16560 (2019). PubMed: 31350351
- Tang XM et al. Upregulation of suppressor of cytokine signaling 3 ameliorates spinal degenerative disease in adolescents by mediating leptin and tumor necrosis factor-a levels. Exp Ther Med 18:2231-2237 (2019). PubMed: 31410173
- Chu G et al. Leptin Receptor Mediates Bmal1 Regulation of Estrogen Synthesis in Granulosa Cells. Animals (Basel) 9:N/A (2019). PubMed: 31683864
- van de Looij Y et al. Nutritional Intervention for Developmental Brain Damage: Effects of Lactoferrin Supplementation in Hypocaloric Induced Intrauterine Growth Restriction Rat Pups. Front Endocrinol (Lausanne) 10:46 (2019). PubMed: 30800096