Anti-Leptin 抗体 (ab7208)

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Sandwich ELISA - Anti-Leptin antibody (ab7208)

    Key features and details

    • Rabbit polyclonal to Leptin
    • Suitable for: Sandwich ELISA
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Leptin antibody
      Leptin 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Leptin

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: Sandwich ELISAmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant full length protein (Human).

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab7208 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use a concentration of 0.5 µg/ml.

    For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab20101 as Capture.

    ターゲット情報

    • 機能

      May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

    • 関連疾患

      Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

    • 配列類似性

      Belongs to the leptin family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • FLJ94114 antibody
        • LEP antibody
        • LEP_HUMAN antibody
        • LEPD antibody
        • Leptin (murine obesity homolog) antibody
        • Leptin (obesity homolog, mouse) antibody
        • Leptin antibody
        • Leptin Murine Obesity Homolog antibody
        • Leptin Precursor Obesity Factor antibody
        • OB antibody
        • Obese protein antibody
        • Obese, mouse, homolog of antibody
        • Obesity antibody
        • Obesity factor antibody
        • Obesity homolog mouse antibody
        • Obesity Murine Homolog Leptin antibody
        • OBS antibody
        • OTTHUMP00000212285 antibody
        see all

      画像

      参考文献 (1)

      ab7208 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab7208 は 1 報の論文で使用されています。

      • Xie X  et al. Characterization of the Human Adipocyte Proteome and Reproducibility of Protein Abundance by One-Dimensional Gel Electrophoresis and HPLC-ESI-MS/MS. J Proteome Res 9:4521-34 (2010). PubMed: 20812759

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      Question

      Good morning,
      I would like to set up a sandwich immunoassay for leptin, employing either HRP or fluorescent nanospheres (prepared in my lab) for detection.
      Do you think I could couple the AB6055 raised in mouse (to be used as a detection antibody) with the AB7208 raised in rabbit (to be used as a capture antibody)?
      If so, do you think I could also employ my nanosphere-labeled anti-mouse secondary antibody to bind to AB6055 instead of using HRP for detection?
      Sincerely yours,

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      Answer

      Thank you for contacting us.

      The HRP conjugated anti-Leptin ab6055 has been tested in sELISA with ab6057 (https://www.abcam.com/ab6057). We do not have any data for the pair ab6055 / ab7208.

      I would not recommend to use a secondary to detect a conjugated antibody.

      Here is the list of the two alternative antibody pairs used in sELISA for the detection of Human Leptin that may be suitable for your work:

      ab20101 / ab7208. Both antibodies are unconjugated: https://www.abcam.com/ab20101 and https://www.abcam.com/ab7208.
      ab6056 / ab36498. Both antibodies are unconjugated: https://www.abcam.com/ab6056 and https://www.abcam.com/ab36498.



      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      Hello,

      Weneedtwo different types ofanti-leptin antibodies for human milk samples, that each ones recognize different epitopes; and a third antibody anti-Ig against the animal where the anti-leptin antibodies were produced. Wich of yours products do you recomend me? We'd like to know exactly the price of each product, the quantity/volume,how long will it take to arrive to Mallorca and if it's only for investigation because we want to introduce it in a commercial instrument.

      I'm waiting for your answer. Thanks

      Read More
      Answer

      Thank you for contacting us and your interest in our products.

      We have a number of different antibodies directed against the human Leptin protein. The ideal choices for your experiment would depend on what application you are hoping to use.

      We have several antibodies which are able to detect different epitopes from the Leptinprotein such as ab50424 which is a goat polyclonal raised againsta synthetic peptide derived from the residues 829-841 of human Leptin. This antibody has been used in ELISA and western blotting.

      The antibodyab20101 (mouse monoclonal)has been raised against the peptide taken from residues 131-145 of human Leptin. This antibody has been used in sandwich ELISA, Western blotting and IHC. The antibody ab20101 was used as an ELISA sandwich pair with the rabbit polyclonal ab7208 which was raised against the full length human Leptin protein. The standard curve was performed using the recombinantly expressed human Leptinprotein (ab51273).

      In order to advise which secondary antibody (anti-IgG of host animal) would be suitable, I would need to know which conjugate you would like to use in your assay. It would also be helpful to know the application in which you wish to use the antibodies. If you could provide me with this information I will be able to advise you further. If you could let me knowwhich antibodies you'd be interested in I can also let you know the delivery time and how much antibody would be provided (alternatively this information isavailable on the datasheet of each product).

      All of our products are for researchpurposes only and are not intended for diagnostic, therapeutic or in vivo use.

      I hope this information has been of some help. If you require any further information please do not hesitate to contact us again.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com