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  1. Link

    leptin-antibody-ab3583.pdf

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Anti-Leptin 抗体 (ab3583)

  • Datasheet
Reviews (3)Q&A (15)References (21)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Leptin antibody (ab3583)
  • Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)
  • Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)
  • Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)
  • Immunohistochemistry (Frozen sections) - Anti-Leptin antibody (ab3583)
  • Flow Cytometry - Anti-Leptin antibody (ab3583)

Key features and details

  • Rabbit polyclonal to Leptin
  • Suitable for: ICC/IF, IHC-Fr, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Sheep, Human, Bird
  • Isotype: IgG

こちらの製品もご検討ください

アッセイ
Product image
Triglyceride Assay Kit - Quantification  (ab65336)

関連製品

製品の概要

  • 製品名

    Anti-Leptin antibody
    Leptin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Leptin
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, IHC-Fr, WB, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Sheep, Human, Bird
    交差が予測される動物種: Goat, Horse, Chicken, Cow, Cat, Dog, Turkey, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse Leptin aa 25-44.
    Sequence:

    QKVQDDTKTLIKTIVTRIND

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • ウェスタン・ブロット用ポジティブコントロールRecombinant Human Leptin protein
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Neurology process
    • Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Leptin protein (ab51273)
    • BALB/3T3 whole cell lysate (ab7901)

アプリケーション

Our Abpromise guarantee covers the use of ab3583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/50 - 1/200.
WB 1/200 - 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
Flow Cyt Use 3-5µl for 106 cells.

ターゲット情報

  • 機能

    May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • 関連疾患

    Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 配列類似性

    Belongs to the leptin family.
  • 細胞内局在

    Secreted.
  • Target information above from: UniProt accession P41159 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 493838 Cat
    • Entrez Gene: 373955 Chicken
    • Entrez Gene: 449638 Chimpanzee
    • Entrez Gene: 280836 Cow
    • Entrez Gene: 3952 Human
    • Entrez Gene: 16846 Mouse
    • Entrez Gene: 25608 Rat
    • Entrez Gene: 698728 Rhesus monkey
    • Omim: 164160 Human
    • SwissProt: Q9N2C1 Cat
    • SwissProt: O42164 Chicken
    • SwissProt: O02750 Chimpanzee
    • SwissProt: P50595 Cow
    • SwissProt: Q257X2 Goat
    • SwissProt: Q9TU09 Horse
    • SwissProt: P41159 Human
    • SwissProt: P41160 Mouse
    • SwissProt: P50596 Rat
    • SwissProt: Q28504 Rhesus monkey
    • SwissProt: Q28603 Sheep
    • Unigene: 194236 Human
    • Unigene: 277072 Mouse
    • Unigene: 44444 Rat
    see all
  • 別名

    • FLJ94114 antibody
    • LEP antibody
    • LEP_HUMAN antibody
    • LEPD antibody
    • Leptin (murine obesity homolog) antibody
    • Leptin (obesity homolog, mouse) antibody
    • Leptin antibody
    • Leptin Murine Obesity Homolog antibody
    • Leptin Precursor Obesity Factor antibody
    • OB antibody
    • Obese protein antibody
    • Obese, mouse, homolog of antibody
    • Obesity antibody
    • Obesity factor antibody
    • Obesity homolog mouse antibody
    • Obesity Murine Homolog Leptin antibody
    • OBS antibody
    • OTTHUMP00000212285 antibody
    see all

画像

  • Western blot - Anti-Leptin antibody (ab3583)
    Western blot - Anti-Leptin antibody (ab3583)

    ab3583 at a dilution of 1 / 4000 staining leptin in mouse 3T3-L1 cell lysate by Western blot.

  • Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)
    Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)

    ab3583 staining Leptin in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml in 0.1% BSA) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody (1/2000) (Panel a). Nuclei were stained with DAPI (Panel b). F-actin stained with Rhodamine Phalloidin (panel c). Merged images shown in panel d. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)
    Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)
    ICC/IF image of ab3583 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3583, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)
    Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)This image is courtesy of an Abreview submitted by Steven Johnstone

    ab3583 staining Leptin in Human mesenchyml stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% horse serum) for 1 hour at 22°C. A FITC-conjugated Mouse anti-rabbit IgG1 monoclonal was used as the secondary antibody (1/100).

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Leptin antibody (ab3583)
    Immunohistochemistry (Frozen sections) - Anti-Leptin antibody (ab3583)

    Immunohistochemical staining of Leptin in Sheep brain using ab3583.

  • Flow Cytometry - Anti-Leptin antibody (ab3583)
    Flow Cytometry - Anti-Leptin antibody (ab3583)

    ab3583 staining Leptin in 3T3-L1 cells by Flow Cytometry. The sample was incubated with the primary antibody (3ug/ml in 2.5% BSA) for 2 hours at room temperature. An Alexa Fluor 488®-conjugated Goat anti-rabbit (1/400) was used as the secondary antibody. Red histogram represents ab3583, pink histogram represents isotype control, purple histogram represents unstained control, green histogram represents no primary antibody control

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (21)

    ab3583 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3583 は 21 報の論文で使用されています。

    • Cao HJ  et al. Effect and mechanism of psoralidin on promoting osteogenesis and inhibiting adipogenesis. Phytomedicine 61:152860 (2019). PubMed: 31048126
    • Tang XM  et al. Upregulation of suppressor of cytokine signaling 3 ameliorates spinal degenerative disease in adolescents by mediating leptin and tumor necrosis factor-a levels. Exp Ther Med 18:2231-2237 (2019). PubMed: 31410173
    • Zhang T  et al. Leptin Expression in Human Epicardial Adipose Tissue Is Associated with Local Coronary Atherosclerosis. Med Sci Monit 25:9913-9922 (2019). PubMed: 31872802
    • Han YC  et al. Leptin regulates disc cartilage endplate degeneration and ossification through activation of the MAPK-ERK signalling pathway in vivo and in vitro. J Cell Mol Med N/A:N/A (2018). WB . PubMed: 29372627
    • Liu Y  et al. HN1L Promotes Triple-Negative Breast Cancer Stem Cells through LEPR-STAT3 Pathway. Stem Cell Reports 10:212-227 (2018). PubMed: 29249663
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 18 Abreviews or Q&A

    Western blot abreview for Anti-Leptin antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Spermophilus tridecemlineatus Tissue lysate - whole (white adipose tissue)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (12.5)
    Loading amount
    20 µg
    Specification
    white adipose tissue
    Blocking step
    BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 05 2014

    Question


    Inquiry: I have got this product (Rabbit polyclonal leptin (ab3583) to detect the level of human Leptin by WB. I need now to use this antibody to block the function of Leptin in human cells. Can I use the same antibody for such purposes?? your help is highly appreciated.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Thank you for contacting us. This antibody has not been tested for blocking functional activity. If you do choose to test it this we way encourage you to submit a review to let us and our customers know whether it worked for you in functional studies.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 10 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Leptin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Mesenchymal stem cell)
    Specification
    Mesenchymal stem cell
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More

    Mr. Steven Johnstone

    Verified customer

    投稿 Sep 05 2012

    Question

    Dear All,
    a customer of our is building a study to compare healty and obese patients.
    She is interested in using your ab3583 and ab16227 on human chondrocyte.
    Do you have any experitise with those Abs on this specific samples? In particular the customer would like to know if all the leptin is secreted or she can detect the leptin also in cellular lysate (she remove the cell supernatant) with your Abs?

    Thanks in advance
    and kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 09 2012

    Answer

    Thank you for your enquiry.

    I can confirm that ab3583 and ab16227 antibodiesshould detect leptin both intra and extracellularlly.However based on the references for these product it appears that most if not all of the leptin is immediately secreted and would therefore be found extracellularlly.

    I am sorry that as far as we are aware, these antibodies have not specifically been tested inchondrocyts.However, I would like to reassure you that our antibodies are tested and covered by our 6 month guarantee in the listed tested applications and species on the individual datasheets.

    I hope this information is helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Jul 09 2012

    Question

    Regarding your answer; i would like to add this comments

    1/Lysis buffer. To extract the protein from my tissue; i have used Qproteome kit from Qiagen, protocol for tissue, with protease inhibitors and benzonase nuclease;

    2/ Loading amount:
    Try to load at least 25-30 ug total tissue lysate per lane onto the gel. I have already load 40ug of total protein. Do you means that i need to load less?

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Mar 23 2012

    Answer

    Thank you for passing some information to me.
    I understand that ab3583 has been used previously at 1:4000.
    Have you had a chance to test this antibody at dilutions of 1/500 or 1/1000 (for 1 hr at room temperature or overnight at 4oC?
    Would you prefer to get a new vial of the primary antibody? Please do let me know how you wish to proceed.
    I look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    Answered on Mar 23 2012

    Question

    I was not working yesterday and I completely forgot to answer to you.

    - species: sheep
    - cell or tissue types: cardiac tissue
    - lysate (total cell lysates, or cytoplasmic fraction etc): lysate of total protein from sheep cardiac tissue.

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Mar 22 2012

    Answer

    Thank you for getting back to me and for specifying the sample types.
    As you have kindly explained to me in your previous e-mail, the primary and the secondary antibodies worked with the positive control but the signal was very weak using lysates from sheep cardiac tissues.
    This antibody (ab3583) should recognize sheep Leptin, the cross-reactivity with this species has been tested and confirmed.
    After reading through the detailed protocol you kindly forwarded to Abcam, I would like to make the following comments/suggestions:
    1) Lysis buffer:
    Leptin is secreted so potentially it can be degraded very quickly. It is very important to prepare the samples from fresh tissues and use lysis buffer which contains proteinase inhibitors. The whole process should be carried out on ice. I understand from the initial e-mail that Laemmli buffer was used for lysing the samples. I would rather suggest using RIPA buffer to prevent any unwanted protein degradation in the sheep cardiac tissues. The recipe of RIPA can be found at this website:
    https://www.abcam.com/index.html?pageconfig=resource&rid=11379#A1
    2) Loading amount:
    Try to load at least 25-30 ug total tissue lysate per lane onto the gel.
    3) Dilution:
    You may need to optimize the dilutions of the primary antibody to find out the most suitable working dilution range for sheep cardiac tissue lysate. Brain tissue has high levels of leptin but cardiac tissue may have significantly lower. I have tried to find some information for you regarding the expressed levels of leptin in sheep tissue, but unfortunately not too much data are available on the public sites compared to human, rat our mouse (i.e. Swiss-Prot, Unigen).
    http://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Oar&CID=524
    http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Rn.44444
    The expression levels of leptin may vary from cell type to cell type or from tissue to tissue. It would be worth testing 1/500 or 1/1000 (for 1 hr at room temperature or overnight at 4oC).
    I hope this will be useful for you. Should you still have any problem with this antibody after following these suggestions, then please do not hesitate to contact me again. I would be delighted to help you further.
    Have a nice day!

    Read More

    Abcam Scientific Support

    Answered on Mar 22 2012

    Question

    Here are the order details for these 2 products:





    Cat#



    Abcam Order Ref#



    Abcam Invoice #



    Sapphire PO#





    ab13987



    1036404



    1312962



    PO-13232





    ab97051



    975213



    1294594-SAP002



    PO-12209





    Please let me know if you require any other information.

    Kind Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    Thank you for confirming the order dfetails.
    Would you be so kind to clarify what samples you have been using:
    - species,
    - cell or tissue types,
    - lysate (total cell lysates, or cytoplasmic fraction etc)
    I look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    Answered on Mar 21 2012

    Question


    See below the answer to your question :
    Q1: Could you please confirm the batch numbers of ab13987 and ab97051?
    ab13987 : GR65976-1
    ab97051 : GR44808-2
    - Q2: Were these products shipped in the same package?
    Yes I think so
    - Q3: Could you please provide the Abcam Order Number(s) or your PON?
    No we bought them from Sapphirebioscience in Australia, one of my
    colleague ordered them, i have not these details.
    - Q4: Have you optimized the dilutions of the primary and the
    secondary antibody?
    I did a dot blot to optimise the secondary dilution because the
    recommended dilution of the primary (found in the datasheet) is
    1:4000. For this dot blot instead of the 1:4000 dilution, I did a
    mistake and use 1:800 dilution. The dot of protein was 300ng, 200 ng,
    150ng, 100ng, 75 ng, 50 ng, 25 ng, 12.5ng. For the secondary the
    dilution was 1:10000, 1:20000, 1:30000, 1:40000 and 1:50000. After a 2
    min exposure, i was able to detect the 12,5 ng of protein with a
    secondary dilution of 1:10000 and 1:20000; with the other secondary
    dilution, i can detect 25 ng of protein (spot is very light) and 50
    ng of protein with the same intensity as the spot obtained with 12,5
    ng of protein with a secondary dilution of 1:10000 and 1:20000. Note
    that the chemiluminescent detection system used for the dot blot was
    more sensitive that the one used for the sensitivity test.
    - Q5: Is the HRP still active (ab97051)? Have you used it successfully
    with another primary antibody?
    I think the antibody is still active because I used it beginning of
    march for the dot blot and a week after to do a first western blot
    with my protein lysate.
    What i have done with this is antibody is:
    1- the dot blot as describe above.
    2- a western blot with my protein lysate (40ug total protein per lane)
    with ab13987 as positive control (300ng), 1:800 dilution for the
    primary antibody (I found out my mistake after the incubation),
    1:20000 for the secondary (ab97051). I can't detect any band in my
    protein lysate but i detected the positive control.
    So we decided to do a sensitivity test to know what is the lower
    amount of protein that the antibody is able to detect.
    3- the sensitivity test
    I hope to hear from you soon
    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Mar 20 2012

    Answer

    Thank you for your prompt answers. Your co-operation in this matter is highly appreciated.
    I understand that you have already carried out some optimization steps and it seems that the primary and the secondary antibodies worked on positive control but the signal was very weak on the samples. Is that correct? Would you be so kind to clarify what samples you have been using:
    - species,
    - cell or tissue types,
    - lysate (total cell lysates, or cytoplasmic fraction etc)
    I have forwarded your response to our Australian Distributor (Sapphire) to get some confirmation about the shipment and order details. Could you please laise with them to find out the Abcam Order number?
    Once again, thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

    Read More

    Abcam Scientific Support

    Answered on Mar 20 2012

    Question

    LOT NUMBER GR16544-2 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal or weak signal i did a sensitivity test and i can not detect concentration of leptin <100ng with the antibody SAMPLE recombinant protein ab13987 PRIMARY ANTIBODY ab 3583, dilution 1:4000 in TBST- 5% milk, 1 hour,room temperature Wash step : 4 washing in TBST for 5 min DETECTION METHOD Chemiluminescence : Immunstar HRP chemiluminescent kit from BIORAD Fluorescent ANTIBODY STORAGE CONDITIONS 10ul aliquot, -20C SAMPLE PREPARATION Laemmli buffer 5 min boil AMOUNT OF PROTEIN LOADED 300ng, 100 ng, 30 ng, 10 ng, 3 ng, 1 ng, 0.3 ng, 0.1 ng, 0.03 ng, 0.01 ng ELECTROPHORESIS/GEL CONDITIONS 18% polyacrylamide gel (BIORAD), TGS, 55 min, 200V TRANSFER AND BLOCKING CONDITIONS transfer : nitrocellulose 0.22 um, 30min, tris-glycine + 20% methanol, blocking : TBST 5% non fat dry milk SECONDARY ANTIBODY 1/goat polyclonal secondary antibody to rabbit IgG ab97051, dilution 1:20000 in TBST+5% milk, 1 hour room temperature Wash step :4 washing in TBST for 5 min 2/1:10000 dilution of anti rabbit IgG coupled with 800 nm fluorochrome Wash step :4 washing in TBST for 5 min HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 ADDITIONAL NOTES the results using chemiluminescent or fluorescent are similar

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    Abcam community

    Verified customer

    Asked on Mar 19 2012

    Answer

    Thank you for enquiry and for taking the time to provide some useful details of the experiments. As I understand three of our products (ab3583, ab13987 and ab97051) have been used in these studies.
    Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem.
    - Q1: Could you please confirm the batch numbers of ab13987 and ab97051?
    - Q2: Were these products shipped in the same package?
    - Q3: Could you please provide the Abcam Order Number(s) or your PON?
    - Q4: Have you optimized the dilutions of the primary and the secondary antibody?
    - Q5: Is the HRP still active (ab97051)? Have you used it successfully with another primary antibody?
    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

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    Abcam Scientific Support

    Answered on Mar 19 2012

    Question

    Dear Sir or Madam,

    A customer bought your ab3583

    The datasheet/vial states that the vial contains 50 ul, however, only 15ul was actually included, even after a spin.

    The vial was not damaged, and the lid was properly fastened.

    Please send a replacement vial for this customer.


    Have a nice day!



    Best regards,

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    Abcam community

    Verified customer

    Asked on Jan 17 2012

    Answer

    Thank you for contacting us.

    I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience.We will be pleased to issue a free of charge replacement vial. In order to do this, I am forwarding these details on to our distributors team who will be happy to organize this for you.

    Thank you for your help and cooperation with this case. Please do not hesitate to contact us if you need anything further.

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    Abcam Scientific Support

    Answered on Jan 17 2012

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