Anti-Leptin 抗体 (ab3583)
Key features and details
- Rabbit polyclonal to Leptin
- Suitable for: ICC/IF, IHC-Fr, WB, Flow Cyt
- Reacts with: Mouse, Rat, Sheep, Human, Bird
- Isotype: IgG
製品の概要
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製品名
Anti-Leptin antibody
Leptin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Leptin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Fr, WB, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Sheep, Human, Bird
交差が予測される動物種: Goat, Horse, Chicken, Cow, Cat, Dog, Turkey, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Mouse Leptin aa 25-44.
Sequence:QKVQDDTKTLIKTIVTRIND
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ポジティブ・コントロール
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab3583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | Use a concentration of 5 µg/ml. | |
IHC-Fr | 1/50 - 1/200. | |
WB | 1/200 - 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). | |
Flow Cyt | Use 3-5µl for 106 cells. |
ターゲット情報
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機能
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
関連疾患
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
配列類似性
Belongs to the leptin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 493838 Cat
- Entrez Gene: 373955 Chicken
- Entrez Gene: 449638 Chimpanzee
- Entrez Gene: 280836 Cow
- Entrez Gene: 3952 Human
- Entrez Gene: 16846 Mouse
- Entrez Gene: 25608 Rat
- Entrez Gene: 698728 Rhesus monkey
see all -
別名
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
see all
画像
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ab3583 at a dilution of 1 / 4000 staining leptin in mouse 3T3-L1 cell lysate by Western blot.
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ab3583 staining Leptin in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml in 0.1% BSA) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody (1/2000) (Panel a). Nuclei were stained with DAPI (Panel b). F-actin stained with Rhodamine Phalloidin (panel c). Merged images shown in panel d.
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ICC/IF image of ab3583 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3583, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)This image is courtesy of an Abreview submitted by Steven Johnstone
ab3583 staining Leptin in Human mesenchyml stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% horse serum) for 1 hour at 22°C. A FITC-conjugated Mouse anti-rabbit IgG1 monoclonal was used as the secondary antibody (1/100).
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Immunohistochemical staining of Leptin in Sheep brain using ab3583.
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ab3583 staining Leptin in 3T3-L1 cells by Flow Cytometry. The sample was incubated with the primary antibody (3ug/ml in 2.5% BSA) for 2 hours at room temperature. An Alexa Fluor 488®-conjugated Goat anti-rabbit (1/400) was used as the secondary antibody. Red histogram represents ab3583, pink histogram represents isotype control, purple histogram represents unstained control, green histogram represents no primary antibody control
プロトコール
データシートおよび資料
参考文献 (21)
ab3583 は 21 報の論文で使用されています。
- Cao HJ et al. Effect and mechanism of psoralidin on promoting osteogenesis and inhibiting adipogenesis. Phytomedicine 61:152860 (2019). PubMed: 31048126
- Tang XM et al. Upregulation of suppressor of cytokine signaling 3 ameliorates spinal degenerative disease in adolescents by mediating leptin and tumor necrosis factor-a levels. Exp Ther Med 18:2231-2237 (2019). PubMed: 31410173
- Zhang T et al. Leptin Expression in Human Epicardial Adipose Tissue Is Associated with Local Coronary Atherosclerosis. Med Sci Monit 25:9913-9922 (2019). PubMed: 31872802
- Han YC et al. Leptin regulates disc cartilage endplate degeneration and ossification through activation of the MAPK-ERK signalling pathway in vivo and in vitro. J Cell Mol Med N/A:N/A (2018). WB . PubMed: 29372627
- Liu Y et al. HN1L Promotes Triple-Negative Breast Cancer Stem Cells through LEPR-STAT3 Pathway. Stem Cell Reports 10:212-227 (2018). PubMed: 29249663