Anti-Legumain 抗体 (ab232870)
Key features and details
- Rabbit polyclonal to Legumain
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Legumain antibody
Legumain 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Legumain -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Macaque monkey, Orangutan -
免疫原
Recombinant full length protein (His-T7-tag) corresponding to Human Legumain aa 1 to the C-terminus. N-terminal tags. Expressed in E. coli. Full length mature chain including propeptide, not including signal peptide.
Database link: Q99538 -
ポジティブ・コントロール
- WB: Recombinant human Legumain protein and rat placenta tissue. IHC-P: Human breast cancer, kidney, stomach, colorectal cancer, prostate and prostate cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab232870の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 49 kDa.
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特記事項 |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 49 kDa. |
ターゲット情報
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機能
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. -
組織特異性
Ubiquitous. Particularly abundant in kidney, heart and placenta. -
配列類似性
Belongs to the peptidase C13 family. -
翻訳後修飾
Glycosylated. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 5641 Human
- Entrez Gene: 100173793 Orangutan
- Entrez Gene: 63865 Rat
- Omim: 602620 Human
- SwissProt: Q99538 Human
- SwissProt: Q5R5D9 Orangutan
- SwissProt: Q9R0J8 Rat
- Unigene: 726036 Human
see all -
別名
- AEP antibody
- Asparaginyl endopeptidase antibody
- cysteine 1 antibody
see all
画像
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Anti-Legumain antibody (ab232870) at 1 µg/ml + Rat placenta tissue lysate
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 49 kDa -
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Legumain using ab232870 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Anti-Legumain antibody (ab232870) at 1 µg/ml + Recombinant human Legumain protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 49 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab232870 は論文での使用が確認できていません。