Anti-Lactate Dehydrogenase 抗体 [EPR1564] - BSA and Azide free (ab226016)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1564] to Lactate Dehydrogenase - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Lactate Dehydrogenase antibody [EPR1564] - BSA and Azide free
Lactate Dehydrogenase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1564] to Lactate Dehydrogenase - BSA and Azide free -
由来種
Rabbit -
特異性
The immunogen for this antibody has 93% homology with L-lactate dehydrogenase C chain (P07864).
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アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab226016 is the carrier-free version of ab101562.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1564 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab226016の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is strongly recommended. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is strongly recommended. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. |
ターゲット情報
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パスウェイ
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
関連疾患
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
配列類似性
Belongs to the LDH/MDH superfamily. LDH family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3939 Human
- Entrez Gene: 3945 Human
- Entrez Gene: 16828 Mouse
- Entrez Gene: 16832 Mouse
- Entrez Gene: 24533 Rat
- Entrez Gene: 24534 Rat
- Omim: 150000 Human
- Omim: 150100 Human
see all -
別名
- Cell proliferation-inducing gene 19 protein antibody
- GSD11 antibody
- L lactate dehydrogenase B chain antibody
see all
画像
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Staining of Human Lactate Dehydrogenase in a Formalin/PFA-fixed paraffin-embedded section of human Muscle using ab101526 at a dilution of 1/500.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101562).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (6)
ab226016 は 6 報の論文で使用されています。
- Wang Z et al. Short-form RON (sf-RON) enhances glucose metabolism to promote cell proliferation via activating ß-catenin/SIX1 signaling pathway in gastric cancer. Cell Biol Toxicol 37:35-49 (2021). PubMed: 32399910
- Ye J et al. The effect of hypoxia on the proliferation capacity of dermal papilla cell by regulating lactate dehydrogenase. J Cosmet Dermatol 20:684-690 (2021). PubMed: 32599679
- Li Y et al. circ_0136666 Facilitates the Progression of Colorectal Cancer via miR-383/CREB1 Axis. Cancer Manag Res 12:6795-6806 (2020). PubMed: 32821160
- Dong C et al. CircSMARCA5 Facilitates the Progression of Prostate Cancer Through miR-432/PDCD10 Axis. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32407167
- Wang X et al. Circular RNA NOX4 promotes the development of colorectal cancer via the microRNA-485-5p/CKS1B axis. Oncol Rep 44:2009-2020 (2020). PubMed: 32901890
- Ju C et al. Bupivacaine suppresses the progression of gastric cancer through regulating circ_0000376/miR-145-5p axis. BMC Anesthesiol 20:275 (2020). PubMed: 33126850