LDH Assay Kit (Cytotoxicity) (ab65393)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media
製品の概要
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製品名
LDH Assay Kit (Cytotoxicity)
Lactate Dehydrogenase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture media -
アッセイタイプ
Enzyme activity (quantitative) -
全工程の試験時間
1h 00m -
製品の概要
LDH Assay Kit (Cytotoxicity) ab65393 uses WST for the fast and sensitive detection of LDH released from damaged cells.
The LDH assay, also known as LDH release assay, is a cell death / cytotoxicity assay used to assess the level of plasma membrane damage in a cell population. Lactate dehydrogenase (LDH) is a stable enzyme, present in all cell types, which is rapidly released into the cell culture medium upon damage of the plasma membrane. LDH is the most widely used marker used to run a cytotoxicity assay.
The LDH assay protocol is based on an enzymatic coupling reaction: LDH released from the cell oxidizes lactate to generate NADH, which then reacts with WST to generate a yellow color. The intensity of the generated color correlates directly with the number of lyzed cells.
Only 10µl of culture medium is required for the assay, and thus the background from serum and culture medium is significantly reduced. Cells can be cultured in regular 10% serum containing medium; no reducing serum or special medium is required for the assay.
In addition, since WST is very stable, the reaction can be read multiple times and can be stopped at any time point during the reaction.
LDH activity can be easily quantified by spectrophotometer or plate reader at OD450nm.
LDH assay protocol summary:
- transfer 10µl culture medium into new plate
- add LDH reaction mix and incubate for 30 min at room temp
- analyze with microplate reader -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K313 LDH-Cytotoxicity Colorimetric Assay Kit II. K313-500 is the same size as the 500 test size of ab65393.
Alternative LDH assays
If you would like to use a fluorometric assay, please refer to LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004).
This kit is more sensitive than the colorimetric LDH Cytoxicity Assay Kit ab65391.
To measure LDH activity in sample types such as serum, plasma, and cell lysates, we recommend LDH assay kit ab102526.
Related products and guides to cytotoxicity / cell viability / proliferation assays
Review our cell health assay guide to learn about our other kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 500 tests 10000 tests Lysis Buffer II 1 x 5ml 20 x 5ml LDH Positive Control 1 vial 20 vials LDH Assay Buffer 1 x 50ml 20 x 50ml Stop Solution IV 1 x 5ml 20 x 5ml Substrate Mix 1 vial 20 vials -
研究分野
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パスウェイ
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
関連疾患
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
配列類似性
Belongs to the LDH/MDH superfamily. LDH family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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別名
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
see all
関連製品
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Assay kits
画像
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Bafilomycin A1 (BafA1) toxicity was assessed in Human hepatic (Huh7.5) cells after 24 hours at different concentrations (12.5nm, 25nm, 50nm and 100nm) were administered to cells using LDH cytotoxicity assay kit (ab65393). Cytotoxicity was measured by subtracting LDH content in remaining viable cells from total LDH in untreated controls.
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Jurkat T cells were cultured in 96-well plate in 100 μl of culture medium. LDH Assay was performed using 10μl of culture medium using the WST probe. Low control (white bar); High control (black bar).
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Comparison of WST-1 and INT based LDH assays. 3T3 cells were cultured in a 96-well plate in 100 µl of culture medium. The LDH assay was performed using 10 µl of culture medium using WST-1 (Brown bar) and INT (Green bar) methods. The WST-1 based LDH assay is more stable and sensitive than the INT based method.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (126)
ab65393 は 126 報の論文で使用されています。
- Cen Y et al. APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway. Exp Ther Med 23:157 (2022). PubMed: 35069838
- Schmit T et al. Interferon-? promotes monocyte-mediated lung injury during influenza infection. Cell Rep 38:110456 (2022). PubMed: 35235782
- Chen YH et al. 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside protects against neuronal cell death and traumatic brain injury-induced pathophysiology. Aging (Albany NY) 14:2607-2627 (2022). PubMed: 35314517
- Wang YW et al. HIF-1a-regulated lncRNA-TUG1 promotes mitochondrial dysfunction and pyroptosis by directly binding to FUS in myocardial infarction. Cell Death Discov 8:178 (2022). PubMed: 35396503
- Saeed MEM et al. In Silico and In Vitro Screening of 50 Curcumin Compounds as EGFR and NF-?B Inhibitors. Int J Mol Sci 23:N/A (2022). PubMed: 35409325