Anti-Lck (phospho Y504) 抗体 (ab4901)

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Western blot - Anti-Lck (phospho Y504) antibody (ab4901)

    Key features and details

    • Rabbit polyclonal to Lck (phospho Y504)
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Lck (phospho Y504) antibody
      Lck 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Lck (phospho Y504)

    • 由来種

      Rabbit

    • 特異性

      Hck [pY522] (75% homology), and Lyn [pY508] (83.3% homology) have not been tested.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Recombinant fragment
      交差が予測される動物種: Mouse, Chicken

    • 免疫原

      Synthetic peptide corresponding to Human Lck (phospho Y504). The sequence is conserved in human and mouse. Amino acid number is based on the mature sequence without the initiator methionine.
      Database link: P06239
      (Peptide available as ab5217)

    • ポジティブ･コントロール

      • Full length untagged recombinant human Lck protein.

    • 特記事項


      Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Tyr-505 is located near the carboxyl terminus of Lck and, when phosphorylated, associates intramolecularly with the SH2 domain in the amino-terminal half of the protein. This helps stabilise Lck in a conformation that, biologically, is relatively inactive. In the absence of phosphorylation at Tyr-505, intramolecular binding of the carboxyl terminus to the SH2 domain does not occur, and Lck exhibits increased activity in vivo.

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    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.3
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA

      BSA is IgG and protease free
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Lck, and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phospho-tyrosine (irrespective of the sequence). The final product is generated by affinity chromatography using a Lck-derived peptide that is phosphorylated at tyrosine 505.

    • 一次抗体 備考

      Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Tyr-505 is located near the carboxyl terminus of Lck and, when phosphorylated, associates intramolecularly with the SH2 domain in the amino-terminal half of the protein. This helps stabilise Lck in a conformation that, biologically, is relatively inactive. In the absence of phosphorylation at Tyr-505, intramolecular binding of the carboxyl terminus to the SH2 domain does not occur, and Lck exhibits increased activity in vivo.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab4901の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 60 kDa.
    特記事項
    WB
    Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 60 kDa.

    ターゲット情報

    • 機能

      Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.

    • 組織特異性

      Expressed specifically in lymphoid cells.

    • 関連疾患

      Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.

    • 配列類似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.

    • ドメイン

      The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.

    • 翻訳後修飾

      Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.

    • 細胞内局在

      Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
    • Information by UniProt

    • 参照データベース

      • 製品の状態

        This protein is known to be similar in amino acid sequence to HCK (P08631), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

      • 別名

        • IMD22 antibody
        • LCK antibody
        • Lck p56 antibody
        • LCK proto-oncogene, Src family tyrosine kinase antibody
        • LCK_HUMAN antibody
        • Leukocyte C-terminal Src kinase antibody
        • LSK antibody
        • Lymphocyte cell specific protein tyrosine kinase antibody
        • Lymphocyte cell-specific protein-tyrosine kinase antibody
        • Lymphocyte specific protein tyrosine kinase antibody
        • Membrane associated protein tyrosine kinase antibody
        • Oncogene lck antibody
        • P56 LCK antibody
        • p56(LSTRA) protein tyrosine kinase antibody
        • p56-LCK antibody
        • p56lck antibody
        • pp58 lck antibody
        • pp58lck antibody
        • Protein YT16 antibody
        • Proto oncogene tyrosine protein kinase LCK antibody
        • Proto-oncogene Lck antibody
        • Protooncogene tyrosine protein kinase LCK antibody
        • T cell specific protein tyrosine kinase antibody
        • T cell-specific protein-tyrosine kinase antibody
        • T lymphocyte specific protein tyrosine kinase p56lck antibody
        • Tyrosine-protein kinase Lck antibody
        • YT 16 antibody
        • YT16 antibody
        see all

      画像

      • Western blot - Anti-Lck (phospho Y504) antibody (ab4901)
        Western blot - Anti-Lck (phospho Y504) antibody (ab4901)
        Peptide Competition:  Full length untagged recombinant human Lck protein was added to control cell extract which does not contain Lck and then resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins then were transferred to nitrocellulose and incubated with 0.50 µg/mL ab4901 antibody, following prior incubation with: (1) the appropriately phosphorylated peptide immunogen, (2) the nonphosphorylated peptide corresponding to the peptide immunogen, (3) the non-phosphorylated peptide derived from the corresponding region of Src, (4) a generic phosphotyrosine containing peptide, (5) no peptide, and (6) the phosphorylated peptide derived from the corresponding region of Src. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStar detection method. The data presented here demonstrate that only the phosphopeptide corresponding to this site completely blocks t

      参考文献 (4)

      ab4901 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab4901 は 4 報の論文で使用されています。

      • Raiter A  et al. A novel role for an old target: CD45 for breast cancer immunotherapy. Oncoimmunology 10:1929725 (2021). PubMed: 34104545
      • De Logu F  et al. Migraine-provoking substances evoke periorbital allodynia in mice. J Headache Pain 20:18 (2019). PubMed: 30764776
      • Inoue R  et al. Effect of a neural relay on liver regeneration in mice: activation of serotonin release from the gastrointestinal tract. FEBS Open Bio 8:449-460 (2018). PubMed: 29511622
      • Zhao H  et al. Secretion of shh by a neurovascular bundle niche supports mesenchymal stem cell homeostasis in the adult mouse incisor. Cell Stem Cell 14:160-73 (2014). IF . PubMed: 24506883

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