Anti-Lck 抗体 (ab3885)
Key features and details
- Rabbit polyclonal to Lck
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-Lck antibody
Lck 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Lck -
由来種
Rabbit -
特異性
This antibody was designed to recognise the activated form of Lck and was raised for that purpose against the linker that connects the SH2 domain and the tyrosine kinase domain of the kinase. This linker sits in the SH3 substrate binding site in the inactive kinase. The position of the linker is based on the crystal structure of inactive Src. However, the antibody does not seem to distinguish the active and inactive forms of Lck. In IF, it recognises kinase dead Lck at the same concentration as constitutively active Lck. In western blot, the antibody is highly specific for Lck versus other tyrosine kinases (see blot). Even, if the antibody was specific for active Lck in IF , it would be expected to recognise both active and inactive forms in Western blot. This is because the linker that the antibody recognises is present in both active and inactive Lck, it was just expected to be concealed in native form inactive Lck. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human, African green monkey
交差が予測される動物種: Chicken
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免疫原
Synthetic peptide corresponding to Human Lck aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab13750) -
特記事項
Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). | |
| ICC/IF | 1/200 - 1/500. |
ターゲット情報
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機能
Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3. -
組織特異性
Expressed specifically in lymphoid cells. -
関連疾患
Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
ドメイン
The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation. -
翻訳後修飾
Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity. -
細胞内局在
Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form. - Information by UniProt
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参照データベース
- Entrez Gene: 396460 Chicken
- Entrez Gene: 3932 Human
- Entrez Gene: 16818 Mouse
- Omim: 153390 Human
- SwissProt: P42683 Chicken
- SwissProt: P06239 Human
- SwissProt: P06240 Mouse
- Unigene: 470627 Human
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製品の状態
This protein is known to be similar in amino acid sequence to HCK (P08631), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
別名
- IMD22 antibody
- LCK antibody
- Lck p56 antibody
see all
画像
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Western blot - Anti-Lck antibody (ab3885)All lanes : Anti-Lck antibody (ab3885) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 4 minutes -
Western blot - Anti-Lck antibody (ab3885)All lanes : Anti-Lck antibody (ab3885) at 1/1000 dilution
Lane 1 : COS cells transfected with mouse Fyn
Lane 2 : COS cells transfected with mouse Lck
Predicted band size: 57 kDaWestern blot against Lck using ab3885.
Lane 1: COS cells transfected with mouse Fyn.
Lane 2: COS cells transfected with mouse Lck.
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Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody (ab3885)Confocal immunofluorescence image of a COS cell transfected with a mouse Lck construct and stained with ab3885 at 1/200.
In untransfected cells, some nuclear staining is also visible as well as Lck staining.
プロトコール
参考文献 (6)
ab3885 は 6 報の論文で使用されています。
- Lamm KYB et al. Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome. Elife 7:N/A (2018). PubMed: 29309034
- Singh DK et al. SRC family kinases in hamster spermatozoa: evidence for the presence of LCK. Reproduction 153:655-669 (2017). PubMed: 28250239
- Luo T et al. Lck Inhibits Heat Shock Protein 65-Mediated Reverse Cholesterol Transport in T Cells. J Immunol 197:3861-3870 (2016). PubMed: 27742830
- Cao W et al. CXXC finger protein 1 is critical for T-cell intrathymic development through regulating H3K4 trimethylation. Nat Commun 7:11687 (2016). PubMed: 27210293
- Clayton KL et al. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. J Immunol 192:782-91 (2014). WB . PubMed: 24337741
- Rüder C et al. The tumor-associated antigen EBAG9 negatively regulates the cytolytic capacity of mouse CD8+ T cells. J Clin Invest 119:2184-203 (2009). IHC (PFA fixed) ; Mouse . PubMed: 19620783
