Anti-LAT 抗体 (ab2507)
Key features and details
- Rabbit polyclonal to LAT
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-LAT antibody
LAT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LAT -
由来種
Rabbit -
特異性
Binds to a 36-40 Kd protein in normal mouse lung and heart tissue. A larger size band of approx. 40-45 Kd is seen in human tumour cell lines. -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide:
CHRLPGSYDSTSSDSLYPRGI
, corresponding to amino acids 29-48 of Human LAT. -
ポジティブ･コントロール
- Human K562 and Jurkat cell lines. Mouse lung and heart lysate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. | |
| ICC | 1/300. |
ターゲット情報
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機能
Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. -
組織特異性
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level). -
翻訳後修飾
Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. -
細胞内局在
Cell membrane. Present in lipid rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 27040 Human
- Entrez Gene: 16797 Mouse
- Omim: 602354 Human
- SwissProt: O43561 Human
- SwissProt: O54957 Mouse
- Unigene: 632179 Human
- Unigene: 10280 Mouse
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別名
- 36 kDa phospho tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adaptor protein antibody
see all
画像
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Western blot - Anti-LAT antibody (ab2507)Antibody recognizes approximately 36 KD size protein and its degradation products in mouse normal tissues. However, antibody specifically reacts with a approximately 46 KD protein in human tumor cell lines Jurkatt and K562. This immunoreactive 46 KD protein appears to be more stable than 36 KD immunoreactive protein observed in normal mouse tissue. (Lane 1, Human T cell line Jurkatt. Lane 2, Mouse Lung Lane 3, Human cell line K562 Lane 4, Mouse Heart).
Antibody recognizes approximately 36 KD size protein and its degradation products in mouse normal tissues. However, antibody specifically reacts with a approximately 46 KD protein in human tumor cell lines Jurkatt and K562. This immunoreactive 46 KD protein appears to be more stable than 36 KD immunoreactive protein observed in normal mouse tissue. (Lane 1, Human T cell line Jurkatt. Lane 2, Mouse Lung Lane 3, Human cell line K562 Lane 4, Mouse Heart).
参考文献
ab2507 は 4 報の論文で使用されています。
- Kota DJ et al. TSG-6 produced by hMSCs delays the onset of autoimmune diabetes by suppressing Th1 development and enhancing tolerogenicity. Diabetes 62:2048-58 (2013). WB . PubMed: 23349496
- Zikherman J et al. Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival. Proc Natl Acad Sci U S A 109:E3-12 (2012). WB ; Mouse . PubMed: 22135465
- Levin SE et al. Inhibition of ZAP-70 Kinase Activity via an Analog-sensitive Allele Blocks T Cell Receptor and CD28 Superagonist Signaling. J Biol Chem 283:15419-15430 (2008). WB ; Human . PubMed: 18378687
- Head BP et al. Microtubules and actin microfilaments regulate lipid raft/caveolae localization of adenylyl cyclase signaling components. J Biol Chem 281:26391-9 (2006). PubMed: 16818493
