Anti-LAT 抗体 (ab2507)

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Western blot - Anti-LAT antibody (ab2507)

    Key features and details

    • Rabbit polyclonal to LAT
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-LAT antibody
      LAT 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to LAT

    • 由来種

      Rabbit

    • 特異性

      Binds to a 36-40 Kd protein in normal mouse lung and heart tissue. A larger size band of approx. 40-45 Kd is seen in human tumour cell lines.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse, Human

    • 免疫原

      Synthetic peptide:

      CHRLPGSYDSTSSDSLYPRGI

      , corresponding to amino acids 29-48 of Human LAT.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab2507の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/1000.
    特記事項
    WB
    1/1000.

    ターゲット情報

    • 機能

      Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

    • 組織特異性

      Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).

    • 翻訳後修飾

      Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
      Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.

    • 細胞内局在

      Cell membrane. Present in lipid rafts.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 36 kDa phospho tyrosine adapter protein antibody
        • 36 kDa phospho-tyrosine adapter protein antibody
        • 36 kDa phospho-tyrosine adaptor protein antibody
        • LAT 1 antibody
        • lat antibody
        • LAT_HUMAN antibody
        • LAT1 antibody
        • Linker for activation of T cells antibody
        • Linker for activation of T cells family member 1 antibody
        • linker for activation of T cells, transmembrane adaptor antibody
        • Linker for activation of T-cells family member 1 antibody
        • p36 38 antibody
        • p36-38 antibody
        • pp36 antibody
        see all

      画像

      • Western blot - Anti-LAT antibody (ab2507)
        Western blot - Anti-LAT antibody (ab2507)
        Antibody recognizes approximately 36 KD size protein and its degradation products in mouse normal tissues. However, antibody specifically reacts with a approximately 46 KD protein in human tumor cell lines Jurkatt and K562. This immunoreactive 46 KD protein appears to be more stable than 36 KD immunoreactive protein observed in normal mouse tissue. (Lane 1, Human T cell line Jurkatt. Lane 2, Mouse Lung Lane 3, Human cell line K562 Lane 4, Mouse Heart).
        Antibody recognizes approximately 36 KD size protein and its degradation products in mouse normal tissues. However, antibody specifically reacts with a approximately 46 KD protein in human tumor cell lines Jurkatt and K562. This immunoreactive 46 KD protein appears to be more stable than 36 KD immunoreactive protein observed in normal mouse tissue. (Lane 1, Human T cell line Jurkatt. Lane 2, Mouse Lung Lane 3, Human cell line K562 Lane 4, Mouse Heart).

      参考文献 (4)

      ab2507 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2507 は 4 報の論文で使用されています。

      • Kota DJ  et al. TSG-6 produced by hMSCs delays the onset of autoimmune diabetes by suppressing Th1 development and enhancing tolerogenicity. Diabetes 62:2048-58 (2013). WB . PubMed: 23349496
      • Zikherman J  et al. Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival. Proc Natl Acad Sci U S A 109:E3-12 (2012). WB ; Mouse . PubMed: 22135465
      • Levin SE  et al. Inhibition of ZAP-70 Kinase Activity via an Analog-sensitive Allele Blocks T Cell Receptor and CD28 Superagonist Signaling. J Biol Chem 283:15419-15430 (2008). WB ; Human . PubMed: 18378687
      • Head BP  et al. Microtubules and actin microfilaments regulate lipid raft/caveolae localization of adenylyl cyclase signaling components. J Biol Chem 281:26391-9 (2006). PubMed: 16818493

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