Anti-LASS3 抗体 (ab272552)
Key features and details
- Rabbit polyclonal to LASS3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LASS3 antibody
LASS3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LASS3 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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免疫原
Recombinant fragment corresponding to Human LASS3 aa 50-150.
Database link: Q8IU89 -
ポジティブ･コントロール
- WB: Human skin tissue. IHC-P: Human skin and testis tissue. IF: U-251 MG cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be involved in sphingolipid synthesis or its regulation. -
配列類似性
Contains 1 homeobox DNA-binding domain.
Contains 1 TLC (TRAM/LAG1/CLN8) domain. -
細胞内局在
Nucleus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 204219 Human
- SwissProt: Q8IU89 Human
- Unigene: 662371 Human
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別名
- Ceramide synthase 3 antibody
- CerS3 antibody
- CERS3_HUMAN antibody
see all
画像
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Western blot - Anti-LASS3 antibody (ab272552)Anti-LASS3 antibody (ab272552) at 0.4 µg/ml + Human skin tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS3 antibody (ab272552)Paraffin-embedded formalin-fixed human skin tissue stained for LASS3 using ab272552 at 1/50 dilurion in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS3 antibody (ab272552)Paraffin-embedded formalin-fixed human testis tissue stained for LASS3 using ab272552 at 1/50 dilurion in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-LASS3 antibody (ab272552)PFA fixed, Triton X-100 permeablized U-251 MG cells (human brain glioma cell line) labeling LASS3 (green) using ab272552at 2 ug/ml in ICC/IF analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS3 antibody (ab272552)Paraffin-embedded formalin-fixed human skeletal tissue stained for LASS3 using ab272552 at 1/50 dilution in immunohistochemical analysis. Shows low expression as expected.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272552 は論文での使用が確認できていません。
