Anti-LAP2 alpha 抗体 (ab5162)
Key features and details
- Rabbit polyclonal to LAP2 alpha
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-LAP2 alpha antibody
LAP2 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LAP2 alpha -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment (His-tag) corresponding to Human LAP2 alpha aa 188-693.
-
特記事項
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eu karyotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: PBS, Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
一次抗体 備考
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eu karyotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab5162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/2500. Detects a band of approximately 83 kDa. |
ターゲット情報
-
関連性
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 7112 Human
- Entrez Gene: 21917 Mouse
- Entrez Gene: 25359 Rat
- Omim: 188380 Human
- SwissProt: P42166 Human
- SwissProt: Q61033 Mouse
- SwissProt: Q62733 Rat
-
別名
- Isoforms beta/gamma antibody
- Lamina associated polypeptide 2 antibody
- LAP2 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody (ab5162)ab5162 at a dilution of 1/250 staining LAP 2 alpha in HeLa cells by immunofluorescence.
A = ab5162, 1/250
B = DNA
C = A and B merged
-
Western blot - Anti-LAP2 alpha antibody (ab5162)ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL). ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL).
プロトコール
参考文献 (13)
ab5162 は 13 報の論文で使用されています。
- Qin Z et al. Long noncoding RNA TMPO-AS1 promotes progression of non-small cell lung cancer through regulating its natural antisense transcript TMPO. Biochem Biophys Res Commun 516:486-493 (2019). PubMed: 31230752
- Wada E et al. Deficiency of emerin contributes differently to the pathogenesis of skeletal and cardiac muscles in LmnaH222P/H222P mutant mice. PLoS One 14:e0221512 (2019). PubMed: 31430335
- Khanna R et al. E3 ubiquitin ligase RNF123 targets lamin B1 and lamin-binding proteins. FEBS J 285:2243-2262 (2018). PubMed: 29676528
- Bártová E et al. Depletion of A-type lamins and Lap2a reduces 53BP1 accumulation at UV-induced DNA lesions and Lap2a protein is responsible for compactness of irradiated chromatin. J Cell Biochem N/A:N/A (2018). PubMed: 29923310
- Victor MB et al. Striatal neurons directly converted from Huntington's disease patient fibroblasts recapitulate age-associated disease phenotypes. Nat Neurosci 21:341-352 (2018). PubMed: 29403030
