Anti-LAP1 抗体 [RL13] (ab2737)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)
  • Immunocytochemistry/ Immunofluorescence - Anti-LAP1 antibody [RL13] (ab2737)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)

Key features and details

  • Mouse monoclonal [RL13] to LAP1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-LAP1 antibody [RL13]
    LAP1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [RL13] to LAP1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Other Immunogen Type corresponding to Rat LAP1. This is a Pore complex-lamina fraction isolated from rat liver nuclear envelopes.

  • ポジティブ･コントロール

    • IHC-P: Rat lymph node, breast, colon tissue. ICC: NS-1 cells.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/20 - 1/50.
ICC/IF 1/100.

ターゲット情報

  • 機能

    Required for normal synaptic spine architecture and function. Necessary for DISC1 and GRM5 localization to postsynaptic density complexes and for both N-methyl D-aspartate receptor-dependent and metabotropic glutamate receptor-dependent long term depression.

  • 組織特異性

    Brain-specific. Isoform 3 is ubiquitously expressed.

  • 配列類似性

    Belongs to the LAP (LRR and PDZ) protein family.
    Contains 17 LRR (leucine-rich) repeats.
    Contains 1 PDZ (DHR) domain.

  • 細胞内局在

    Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Densin 180 antibody
      • Densin antibody
      • Densin-180 antibody
      • densin180 antibody
      • DKFZp686I1147 antibody
      • KIAA1365 antibody
      • Lamin associated polypeptide 1 antibody
      • LAP 1 antibody
      • Leucine rich repeat containing 7 antibody
      • Leucine rich repeat containing protein 7 antibody
      • Leucine-rich repeat-containing protein 7 antibody
      • Lrrc 7 antibody
      • LRRC7 antibody
      • LRRC7_HUMAN antibody
      • MGC144918 antibody
      • OTTHUMP00000065336 antibody
      • OTTHUMP00000065337 antibody
      • OTTHUMP00000065338 antibody
      • Protein Lap 1 antibody
      • Protein lap1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)

      Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) of rat lymph node tissue labeling LAP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-LAP1 antibody [RL13] (ab2737)
      Immunocytochemistry/ Immunofluorescence - Anti-LAP1 antibody [RL13] (ab2737)

      Immunocytochemical analysis of NS-1 (Mouse myeloma cell line) cells labeling LAP1 with ab2737 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)

      Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) rat breast tissue labeling LAP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)

      Immunohistochemical analysis of (Formalin/PFA-fixed paraffin-embedded sections) rat colon tissue labeling LAP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

    参考文献 (6)

    ab2737 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2737 は 6 報の論文で使用されています。

    • Liao JJ  et al. Distinctive T cell-suppressive signals from nuclearized type 1 sphingosine 1-phosphate G protein-coupled receptors. J Biol Chem 282:1964-72 (2007). WB . PubMed: 17121832
    • Yang L  et al. Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase. J Cell Biol 139:1077-87 (1997). PubMed: 9382857
    • Guan T  et al. Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol Biol Cell 6:1591-603 (1995). PubMed: 8589458
    • Martin L  et al. cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane. J Biol Chem 270:8822-8 (1995). PubMed: 7721789
    • Foisner R & Gerace L Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell 73:1267-79 (1993). PubMed: 8324822
    • Senior A & Gerace L Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina. J Cell Biol 107:2029-36 (1988). PubMed: 3058715

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