Application
Western blot
Sample
Human Cell lysate - whole cell (SY5Y cell)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
15 µg
Treatment
LAMP2A siRNA for 48hrs
Specification
SY5Y cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in TBST
Dilution
1/2000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Rabbit IgG HRP-conjugated Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 100 kDa
Positive control
my samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jie Zhang
Verified customer
投稿 Apr 09 2021