Anti-LAMP1 抗体 - Lysosome Marker (ab24170)
Key features and details
- Rabbit polyclonal to LAMP1 - Lysosome Marker
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Chicken, Hamster, Cat, Dog, Human, Xenopus laevis, Zebrafish, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-LAMP1 antibody - Lysosome Marker
LAMP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LAMP1 - Lysosome Marker -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Chicken, Hamster, Cat, Dog, Human, Xenopus laevis, Zebrafish, African green monkey
交差が予測される動物種: Cow
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免疫原
Synthetic peptide corresponding to Human LAMP1 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab25744) -
ポジティブ･コントロール
- WB: Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line), HEK293 (Human embryonic kidney cell line) and MCF7 (Human breast adenocarcinoma cell line) whole cell lysates. IHC-P: FFPE human normal kidney tissue sections.
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特記事項
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab24170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 90-130 kDa (predicted molecular weight: 120 kDa). Some variability in MW may be observed due to differing levels of glycosylation of the target protein in different cell/tissue types. Abcam recommends using Milk as the blocking agent. |
ターゲット情報
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機能
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
細胞内局在
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3916 Human
- Entrez Gene: 16783 Mouse
- Entrez Gene: 25328 Rat
- Omim: 153330 Human
- SwissProt: P11279 Human
- SwissProt: P11438 Mouse
- SwissProt: P14562 Rat
- Unigene: 494419 Human
see all -
別名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
画像
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Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an Abreview submitted by Dr. Vladimir MilenkovicAnti-LAMP1 antibody - Lysosome Marker (ab24170) at 1/5000 dilution + Mongolian gerbil brain whole cell lysate at 25 µg
Secondary
Polyclonal goat anti-rabbit HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 3 minutes
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Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml
Lane 1 : Jurkat (Human) Whole Cell Lysate
Lane 2 : HEK293 (Human) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab24170 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)IHC image of LAMP1 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24170, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)Immunohistochemical analysis of dog retina stained for LAMP1 (red) using ab24170 at 1:200, followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Alexa Fluor® 568-conjugated at 1:500. Counterstain: DAPI (blue).
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)Immunohistochemical analysis of cat retina stained for LAMP1 (red) using ab24170 at 1:200, followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Alexa Fluor® 568-conjugated at 1:500. Counterstain: DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an Abreview submitted by Mr Carl Hobbsab24170 staining LAMP1 in human kidney tissue sections. Staining correlates with lysosomal specificity, particularly in the proximal convoluted tubules where lysosomes are enriched. Formalin/PFA-fixed human kidney tissue sections were incubated with ab24170 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Please see accompanying abreview for additional information. -
Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Additional bands at: 23 kDa, 35 kDa, 45 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an abreview submitted by Dr. Martin Broadstock (King's College London, United Kingdom)IHC-P image of LAMP1 staining on human Cortex sections using ab24170 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retreival using citric acid. The sections were then permeabilized using 0.05% Tween-20 and blocking was performed using 3% BSA for 1 hour at 21°C. The primary antibody ab24170 was diluted using 3% BSA with 0.05% Tween-20 in PBS and incubated with the sections for 18 hours at 4°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:500)
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Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an anonymous AbreviewAnti-LAMP1 antibody - Lysosome Marker (ab24170) at 1/700 dilution (in 5% milk for 4 hours at 20°C) + Rat Kidney - whole tissue lysate at 18 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Exposure time: 5 minutes
Blocking Step: 5% Milk for 1 hour at 20°C
参考文献 (426)
ab24170 は 426 報の論文で使用されています。
- Rostami J et al. Astrocytes have the capacity to act as antigen-presenting cells in the Parkinson's disease brain. J Neuroinflammation 17:119 (2020). PubMed: 32299492
- Duan C et al. Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH- pathways. Cell Death Dis 11:251 (2020). PubMed: 32312970
- Wei J et al. TRPV1 activation mitigates hypoxic injury in mouse cardiomyocytes by inducing autophagy through the AMPK signaling pathway. Am J Physiol Cell Physiol 318:C1018-C1029 (2020). PubMed: 32293932
- Hintze S et al. Moss-Derived Human Recombinant GAA Provides an Optimized Enzyme Uptake in Differentiated Human Muscle Cells of Pompe Disease. Int J Mol Sci 21:N/A (2020). PubMed: 32290314
- Lisjak M et al. Indirect Role of AQP4b and AQP4d Isoforms in Dynamics of Astrocyte Volume and Orthogonal Arrays of Particles. Cells 9:N/A (2020). PubMed: 32192013
