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  1. Link

    lamp1-antibody-lysosome-marker-ab24170.pdf

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Anti-LAMP1 抗体 - Lysosome Marker (ab24170)

  • Datasheet
  • SDS
Reviews (89)Q&A (6)References (426)

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Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)

Key features and details

  • Rabbit polyclonal to LAMP1 - Lysosome Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Hamster, Dog, Human, Xenopus laevis, Zebrafish, African green monkey
  • Isotype: IgG

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関連製品

このターゲットにつきましては、リコンビナント・ウサギ・モノクローナル抗体も販売しています

製品の概要

  • 製品名

    Anti-LAMP1 antibody - Lysosome Marker
    LAMP1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to LAMP1 - Lysosome Marker
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Hamster, Dog, Human, Xenopus laevis, Zebrafish, African green monkey
    交差が予測される動物種: Chicken, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to Human LAMP1 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab25744)

  • 特記事項

      

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Hematopoietic Progenitors
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    • B Lymphocytic Lineage
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    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Signal Transduction
    • Antibodies
    • lamp1

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human LAMP1 peptide (ab25744)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human LAMP1 protein (ab151863)
  • Related Products

    • Lysosomal Staining Kit - Blue Fluorescence - Cytopainter (ab112135)
    • Lysosomal Staining Kit - Red Fluorescence - Cytopainter (ab112137)
    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

Our Abpromise guarantee covers the use of ab24170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90-130 kDa (predicted molecular weight: 120 kDa).

Some variability in MW may be observed due to differing levels of glycosylation of the target protein in different cell/tissue types. Abcam recommends using Milk as the blocking agent. 

ターゲット情報

  • 機能

    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • 配列類似性

    Belongs to the LAMP family.
  • 翻訳後修飾

    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • 細胞内局在

    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Target information above from: UniProt accession P11279 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3916 Human
    • Entrez Gene: 16783 Mouse
    • Entrez Gene: 25328 Rat
    • Omim: 153330 Human
    • SwissProt: P11279 Human
    • SwissProt: P11438 Mouse
    • SwissProt: P14562 Rat
    • Unigene: 494419 Human
    • Unigene: 16716 Mouse
    • Unigene: 475822 Mouse
    • Unigene: 40177 Rat
    see all
  • 別名

    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

画像

  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml

    Lane 1 : Jurkat (Human) Whole Cell Lysate
    Lane 2 : HEK293 (Human) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 120 kDa
    Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab24170 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)

    IHC image of LAMP1 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24170, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)

    Immunohistochemical analysis of dog retina stained for LAMP1 (red) using ab24170 at 1:200, followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Alexa Fluor® 568-conjugated at 1:500. Counterstain: DAPI (blue).

    See Abreview

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)

    Immunohistochemical analysis of cat retina stained for LAMP1 (red) using ab24170 at 1:200, followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Alexa Fluor® 568-conjugated at 1:500. Counterstain: DAPI (blue).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an Abreview submitted by Mr Carl Hobbs
    ab24170 staining LAMP1 in human kidney tissue sections. Staining correlates with lysosomal specificity, particularly in the proximal convoluted tubules where lysosomes are enriched. Formalin/PFA-fixed human kidney tissue sections were incubated with ab24170 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Please see accompanying abreview for additional information.

    See Abreview

  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 120 kDa
    Additional bands at: 23 kDa, 35 kDa, 45 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an abreview submitted by Dr. Martin Broadstock (King's College London, United Kingdom)

    IHC-P image of LAMP1 staining on human Cortex sections using ab24170 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retreival using citric acid. The sections were then permeabilized using 0.05% Tween-20 and blocking was performed using 3% BSA for 1 hour at 21°C. The primary antibody ab24170 was diluted using  3% BSA with 0.05% Tween-20 in PBS and incubated  with the sections for 18 hours at 4°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:500)

    See Abreview

  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an anonymous Abreview
    Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1/700 dilution (in 5% milk for 4 hours at 20°C) + Rat Kidney - whole tissue lysate at 18 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 120 kDa


    Exposure time: 5 minutes


    Blocking Step: 5% Milk for 1 hour at 20°C

    See Abreview

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (426)

    ab24170 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab24170 は 426 報の論文で使用されています。

    • Rostami J  et al. Astrocytes have the capacity to act as antigen-presenting cells in the Parkinson's disease brain. J Neuroinflammation 17:119 (2020). PubMed: 32299492
    • Duan C  et al. Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH- pathways. Cell Death Dis 11:251 (2020). PubMed: 32312970
    • Wei J  et al. TRPV1 activation mitigates hypoxic injury in mouse cardiomyocytes by inducing autophagy through the AMPK signaling pathway. Am J Physiol Cell Physiol 318:C1018-C1029 (2020). PubMed: 32293932
    • Hintze S  et al. Moss-Derived Human Recombinant GAA Provides an Optimized Enzyme Uptake in Differentiated Human Muscle Cells of Pompe Disease. Int J Mol Sci 21:N/A (2020). PubMed: 32290314
    • Lisjak M  et al. Indirect Role of AQP4b and AQP4d Isoforms in Dynamics of Astrocyte Volume and Orthogonal Arrays of Particles. Cells 9:N/A (2020). PubMed: 32192013
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 95 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LAMP1 antibody - Lysosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Vero cells)
    Permeabilization
    Yes - Saponin
    Specification
    Vero cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 25 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LAMP1 antibody - Lysosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
    Permeabilization
    Yes - PBST(0.5% v/v triton X-100, sigma)
    Specification
    tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 16 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-LAMP1 antibody - Lysosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Dog Tissue sections (Retina)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% TritonX-100 + 0.05% Tween20
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 27 2020

    Western blot abreview for Anti-LAMP1 antibody - Lysosome Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    50000 cells
    Specification
    Glioblastoma
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 15 2019

    Western blot abreview for Anti-LAMP1 antibody - Lysosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma cells)
    Gel Running Conditions
    Reduced Denaturing (4 - 12%)
    Loading amount
    50000 cells
    Specification
    Glioblastoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 22 2019

    Western blot abreview for Anti-LAMP1 antibody - Lysosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Gerbil Cell lysate - whole cell (Mongolian gerbil brain)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    25 µg
    Specification
    Mongolian gerbil brain
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    投稿 May 27 2019

    Immunohistochemistry free floating abreview for Anti-LAMP1 antibody - Lysosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (PFA fixed Brain)
    Specification
    PFA fixed Brain
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 14 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LAMP1 antibody - Lysosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.3% triton x100
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 09 2018

    IHC - Wholemount abreview for Anti-LAMP1 antibody - Lysosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (intestine organoids)
    Specification
    intestine organoids
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 11 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-LAMP1 antibody - Lysosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (tumor)
    Permeabilization
    No
    Specification
    tumor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 30 2018

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    製品・研究分野別
    • 癌
    • 循環器
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