製品の概要

  • 製品名

    Anti-LAMP1 antibody - Lysosome Marker
    LAMP1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to LAMP1 - Lysosome Marker
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IP, IHC-P, WB, IHC-Frmore details
  • 種交差性

    交差種: Mouse, Rat, Chicken, Hamster, Cat, Dog, Human, Xenopus laevis, Zebrafish, African green monkey
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide corresponding to Human LAMP1 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab25744)

  • ポジティブ・コントロール

    • WB: Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line), HEK293 (Human embryonic kidney cell line) and MCF7 (Human breast adenocarcinoma cell line) whole cell lysates. IHC-P: FFPE human normal kidney tissue sections.
  • 特記事項

      

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration. PubMed: 21152024
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90-130 kDa (predicted molecular weight: 120 kDa).

Some variability in MW may be observed due to differing levels of glycosylation of the target protein in different cell/tissue types. Abcam recommends using Milk as the blocking agent. 

IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • 配列類似性

    Belongs to the LAMP family.
  • 翻訳後修飾

    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • 細胞内局在

    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

画像

  • IHC image of LAMP1 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24170, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml

    Lane 1 : Jurkat (Human) Whole Cell Lysate
    Lane 2 : HEK293 (Human) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 120 kDa
    Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab24170 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • ab24170 staining LAMP1 in human kidney tissue sections. Staining correlates with lysosomal specificity, particularly in the proximal convoluted tubules where lysosomes are enriched. Formalin/PFA-fixed human kidney tissue sections were incubated with ab24170 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Please see accompanying abreview for additional information.

    See Abreview

  • All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 120 kDa
    Additional bands at: 23 kDa, 35 kDa, 45 kDa. We are unsure as to the identity of these extra bands.

  • IHC-P image of LAMP1 staining on human Cortex sections using ab24170 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retreival using citric acid. The sections were then permeabilized using 0.05% Tween-20 and blocking was performed using 3% BSA for 1 hour at 21°C. The primary antibody ab24170 was diluted using  3% BSA with 0.05% Tween-20 in PBS and incubated  with the sections for 18 hours at 4°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:500)

    See Abreview

  • Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1/700 dilution (in 5% milk for 4 hours at 20°C) + Rat Kidney - whole tissue lysate at 18 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 120 kDa


    Exposure time: 5 minutes


    Blocking Step: 5% Milk for 1 hour at 20°C

    See Abreview

参考文献

This product has been referenced in:

  • Cioni JM  et al. Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons. Cell 176:56-72.e15 (2019). Read more (PubMed: 30612743) »
  • Zhou T  et al. Mst1 inhibition attenuates non-alcoholic fatty liver disease via reversing Parkin-related mitophagy. Redox Biol 21:101120 (2019). Read more (PubMed: 30708325) »
See all 363 Publications for this product

レビューと Q&A

1-10 of 98 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50000 cells
Specification
Glioblastoma
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C

Abcam user community

Verified customer

投稿 Aug 15 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Jul 22 2019

Application
Western blot
Sample
Gerbil Cell lysate - whole cell (Mongolian gerbil brain)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Mongolian gerbil brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Herr Dr. Vladimir Milenkovic

Verified customer

投稿 May 27 2019

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (PFA fixed Brain)
Specification
PFA fixed Brain

Abcam user community

Verified customer

投稿 Feb 14 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.3% triton x100
Specification
HeLa
Blocking step
BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Nov 09 2018

Application
IHC - Wholemount
Sample
Mouse Tissue (intestine organoids)
Specification
intestine organoids

Abcam user community

Verified customer

投稿 Jul 11 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (tumor)
Permeabilization
No
Specification
tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Apr 30 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (artery)
Permeabilization
No
Specification
artery
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Apr 30 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

投稿 Nov 06 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (fat)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
fat
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

投稿 Nov 06 2017

1-10 of 98 Abreviews or Q&A

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