Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Raw 264.7 murine macrophage)
Permeabilization
Yes - methanol
Specification
Raw 264.7 murine macrophage
Fixative
Methanol
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Invitrogen Alexa flour 568 anti mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Additional data
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in ICC/IF using mouse samples. Although there are publications stating this antibody works with mouse samples and previous batches gave positive staining on murine cells, recent batches are failing to react with this species, as described in general notes of datasheet. It is possible that it may not be suitable for use of mouse samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
泉 野村
Verified customer
投稿 Nov 07 2016