Western blot abreview for Anti-LAMP1 antibody [H4A3]

Average
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (h9c2)
Specification
h9c2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%MILK
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution
1/4000

Detection

Detection method
Licor scan
Bands
Specific: about 100 kDa
Exposure
5 minute(s) and 0 second(s)

Additional data

Additional Notes
The sample was boiled at 95C for 5 min. The predicted Lamp1 protein is 45KD.But because it is a glycoprotein. Its observed size is around 100KD.

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投稿 Feb 03 2015

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