Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
50 µg
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 2% non-fat milk powder
Dilution
1/10000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: ECL-peroxidaselabelled sheep antimouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: HiLyte Plus™ 647
Host species: Sheep
Clonality: Polyclonal
Conjugation: HiLyte Plus™ 647
Detection
Detection method
ECL advance
Exposure
30 second(s)
Bands
Specific: 120 and 48 kDa
Additional data
Additional Notes
The secondary antibody is conjugated with HRP (Horseradish Peroxidase)
Visualised bands: 120 kDa for the glycosilated form of the protein 48 kDa for a probably unglycosilated form of the protein
PubMed ID
18607068: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. elena sbalchiero
Verified customer
投稿 Sep 02 2008