Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (breast MCF-7)
Permeabilization
Yes - 0.1% TX-100
Specification
breast MCF-7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 0.1% BSA
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse Alexa Fluor 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 12 2008