Name: Anti-LAMP1 antibody [EPR4204]
Brand: RabMAb
SKU: ab108597
Rating: 3 (3 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4204] to LAMP1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

製品名

Anti-LAMP1 antibody [EPR4204]
LAMP1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR4204] to LAMP1
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC or IP
種交差性

交差種: Human
免疫原

Synthetic peptide within Human LAMP1 aa 100-200. The exact sequence is proprietary.
ポジティブ･コントロール
- WB: HeLa, JAR, A431 and Jurkat lysates. IHC-P: human liver carcinoma and kidney tissue.
特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR4204
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)
Compatible Secondaries
- Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human LAMP1 protein (ab151863)

Our Abpromise guarantee covers the use of ab108597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
WB		1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa).
IHC-P		1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

追加情報

Is unsuitable for Flow Cyt,ICC or IP.

機能

Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
配列類似性

Belongs to the LAMP family.
翻訳後修飾

O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
細胞内局在

Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Target information above from: UniProt accession P11279 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 3916 Human
- Omim: 153330 Human
- SwissProt: P11279 Human
- Unigene: 494419 Human
別名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
- CD107a antigen antibody
- LAMP 1 antibody
- LAMP-1 antibody
- LAMP1 antibody
- LAMP1_HUMAN antibody
- LAMPA antibody
- LGP120 antibody
- lgpA antibody
- Lysosomal membrane glycoprotein 120KD antibody
- Lysosomal Associated Membrane Protein 1 antibody
- Lysosome associated membrane glycoprotein 1 antibody
- Lysosome-associated membrane glycoprotein 1 antibody
- Lysosome-associated membrane protein 1 antibody
- OTTHUMP00000040663 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LAMP1 with purified ab108597 at 1/400 dilution (0.36 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.

Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)

All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LAMP1 knockout HAP1 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : Human Kidney whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 45 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab108597 observed at 110 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab108597 was shown to recognize LAMP1 in wild-type HAP1 cells as signal was lost at the expected MW in LAMP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LAMP1 knockout samples were subjected to SDS-PAGE. ab108597 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)

All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

The band observed is caused by heavily glycosylation which is consisitent with PMID: 10521503
Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)

All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution ((unpurified))

Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)

Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using unpurified ab108597 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-LAMP1 antibody [EPR4204] (ab108597)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

ab108597 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108597 は 3 報の論文で使用されています。

Sanyal A et al. LRRK2 Kinase Inhibition Rescues Deficits in Lysosome Function Due to Heterozygous GBA1 Expression in Human iPSC-Derived Neurons. Front Neurosci 14:442 (2020). PubMed: 32499675
Yokoi A et al. Mechanisms of nuclear content loading to exosomes. Sci Adv 5:eaax8849 (2019). PubMed: 31799396
Wu X et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . PubMed: 23856606

レビューと Q&A

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レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293)

Gel Running Conditions

Reduced Denaturing (4-20%)

Loading amount

10 µg

Specification

HEK293

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Dec 18 2020

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (10% gel)

Sample

Human Cell lysate - whole cell (Cortical tissue)

Specification

Cortical tissue

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Martin Broadstock

Verified customer

投稿 Jun 03 2013

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (BA9 cortical sections)

Specification

BA9 cortical sections

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Yes - 0.05% Tween-20

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Dr. Martin Broadstock

Verified customer

投稿 May 17 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

ターゲット情報

機能

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

参考文献 (3)

レビューと Q&A

Western blot abreview for Anti-LAMP1 antibody [EPR4204]

Western blot

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LAMP1 antibody [EPR4204]