Anti-LAMP1 抗体 [EPR4204] (ab108597)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)](https://www.abcam.co.jp/ps/products/108/ab108597/Images/ab108597-4-anti-lamp1-antibody-epr4204-immunohistochemistry-liver-carcinoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4204] to LAMP1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LAMP1 antibody [EPR4204]
LAMP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4204] to LAMP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa, JAR, A431 and Jurkat lysates. IHC-P: human liver carcinoma and kidney tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4204 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108597の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa).
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| IHC-P | (1) |
1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa). |
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IHC-P
1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
細胞内局在
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3916 Human
- Omim: 153330 Human
- SwissProt: P11279 Human
- Unigene: 494419 Human
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別名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LAMP1 with purified ab108597 at 1/400 dilution (0.36 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)](https://www.abcam.co.jp/ps/products/108/ab108597/Images/ab108597-304630-anti-lamp1-antibody-epr4204-western-blot.jpg)
Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LAMP1 knockout HAP1 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : Human Kidney whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 45 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab108597 observed at 110 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108597 was shown to recognize LAMP1 in wild-type HAP1 cells as signal was lost at the expected MW in LAMP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LAMP1 knockout samples were subjected to SDS-PAGE. ab108597 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)](https://www.abcam.co.jp/ps/products/108/ab108597/Images/ab108597-365445-anti-lamp1-antibody-epr4204-westernblot-human.jpg)
Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?The band observed is caused by heavily glycosylation which is consisitent with PMID: 10521503
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![Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)](https://www.abcam.co.jp/ps/products/108/ab108597/Images/ab108597-121295-anti-lamp1-antibody-epr4204-western-blot.jpg)
Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution ((unpurified))
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)](https://www.abcam.co.jp/ps/products/108/ab108597/Images/ab108597-121294-anti-lamp1-antibody-epr4204-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using unpurified ab108597 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-LAMP1 antibody [EPR4204] (ab108597)](https://www.abcam.co.jp/ps/products/108/ab108597/Images/ab108597-6-benefits-of-recombinant-antibodies.png)
Anti-LAMP1 antibody [EPR4204] (ab108597)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab108597 は 6 報の論文で使用されています。
- Sanyal A et al. LRRK2 Kinase Inhibition Rescues Deficits in Lysosome Function Due to Heterozygous GBA1 Expression in Human iPSC-Derived Neurons. Front Neurosci 14:442 (2020). PubMed: 32499675
- Okarmus J et al. Lysosomal perturbations in human dopaminergic neurons derived from induced pluripotent stem cells with PARK2 mutation. Sci Rep 10:10278 (2020). PubMed: 32581291
- Kapoor A et al. A simplified aortic ring assay: A useful ex vivo method to assess biochemical and functional parameters of angiogenesis. Matrix Biol Plus 6-7:100025 (2020). PubMed: 33543023
- Sanyal A et al. Lysosome and Inflammatory Defects in GBA1-Mutant Astrocytes Are Normalized by LRRK2 Inhibition. Mov Disord 35:760-773 (2020). PubMed: 32034799
- Yokoi A et al. Mechanisms of nuclear content loading to exosomes. Sci Adv 5:eaax8849 (2019). PubMed: 31799396
- Wu X et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . PubMed: 23856606