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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-LAMP1 抗体 [EPR4204] (ab108597)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (3)

Product price, shipping and contact information

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)
  • Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
  • Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
  • Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)
  • Anti-LAMP1 antibody [EPR4204] (ab108597)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4204] to LAMP1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

こちらの製品もご検討ください

 
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Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
タンパク質
Recombinant Human LAMP1 protein (ab151863)

関連製品

製品の概要

  • 製品名

    Anti-LAMP1 antibody [EPR4204]
    LAMP1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR4204] to LAMP1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide within Human LAMP1 aa 100-200. The exact sequence is proprietary.

  • ポジティブ・コントロール

    • WB: HeLa, JAR, A431 and Jurkat lysates. IHC-P: human liver carcinoma and kidney tissue.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR4204
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Signal Transduction
    • Antibodies
    • lamp1

関連製品

  • Alternative Versions

    • Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
    • Jurkat membrane extract lysate (ab30130)
    • A431 membrane extract lysate (ab30134)
  • Recombinant Protein

    • Recombinant Human LAMP1 protein (ab151863)

アプリケーション

Our Abpromise guarantee covers the use of ab108597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能

      Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
    • 配列類似性

      Belongs to the LAMP family.
    • 翻訳後修飾

      O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
    • 細胞内局在

      Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
    • Target information above from: UniProt accession P11279 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 3916 Human
      • Omim: 153330 Human
      • SwissProt: P11279 Human
      • Unigene: 494419 Human
      • 別名

        • CD107 antigen like family member A antibody
        • CD107 antigen-like family member A antibody
        • CD107a antibody
        • CD107a antigen antibody
        • LAMP 1 antibody
        • LAMP-1 antibody
        • LAMP1 antibody
        • LAMP1_HUMAN antibody
        • LAMPA antibody
        • LGP120 antibody
        • lgpA antibody
        • Lysosomal membrane glycoprotein 120KD antibody
        • Lysosomal Associated Membrane Protein 1 antibody
        • Lysosome associated membrane glycoprotein 1 antibody
        • Lysosome-associated membrane glycoprotein 1 antibody
        • Lysosome-associated membrane protein 1 antibody
        • OTTHUMP00000040663 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LAMP1 with purified ab108597 at 1/400 dilution (0.36 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.

        Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
        Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
        All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution

        Lane 1 : Wild-type HAP1 whole cell lysate
        Lane 2 : LAMP1 knockout HAP1 whole cell lysate
        Lane 3 : MCF7 whole cell lysate
        Lane 4 : Human Kidney whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Predicted band size: 45 kDa
        Observed band size: 110 kDa
        why is the actual band size different from the predicted?



        Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab108597 observed at 110 kDa. Red - loading control, ab18058, observed at 130 kDa.

        ab108597 was shown to recognize LAMP1 in wild-type HAP1 cells as signal was lost at the expected MW in LAMP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LAMP1 knockout samples were subjected to SDS-PAGE. ab108597 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
        Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
        All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution (Purified)

        Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
        Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 45 kDa
        Observed band size: 110 kDa why is the actual band size different from the predicted?



        The band observed is caused by heavily glycosylation which is consisitent with PMID: 10521503

      • Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
        Western blot - Anti-LAMP1 antibody [EPR4204] (ab108597)
        All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution ((unpurified))

        Lane 1 : HeLa cell lysate
        Lane 2 : JAR cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : A431 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 45 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] (ab108597)

        Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using unpurified ab108597 at a dilution of 1/100.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-LAMP1 antibody [EPR4204] (ab108597)
        Anti-LAMP1 antibody [EPR4204] (ab108597)

      プロトコール

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet
      • SDS
    • 参考文献 (3)

      ab108597 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab108597 は 3 報の論文で使用されています。

      • Sanyal A  et al. LRRK2 Kinase Inhibition Rescues Deficits in Lysosome Function Due to Heterozygous GBA1 Expression in Human iPSC-Derived Neurons. Front Neurosci 14:442 (2020). PubMed: 32499675
      • Yokoi A  et al. Mechanisms of nuclear content loading to exosomes. Sci Adv 5:eaax8849 (2019). PubMed: 31799396
      • Wu X  et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . PubMed: 23856606

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-LAMP1 antibody [EPR4204]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK293)
      Gel Running Conditions
      Reduced Denaturing (4-20%)
      Loading amount
      10 µg
      Specification
      HEK293
      Blocking step
      (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Dec 18 2020

      Western blot

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Sample
      Human Cell lysate - whole cell (Cortical tissue)
      Specification
      Cortical tissue
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Dr. Martin Broadstock

      Verified customer

      投稿 Jun 03 2013

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LAMP1 antibody [EPR4204]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (BA9 cortical sections)
      Specification
      BA9 cortical sections
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      Yes - 0.05% Tween-20
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
      Read More

      Dr. Martin Broadstock

      Verified customer

      投稿 May 17 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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