Anti-Laminin alpha 5/LAMA5 抗体 [EPR18919] - BSA and Azide free (ab271947)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free
Laminin alpha 5/LAMA5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5 - BSA and Azide free -
由来種
Rabbit -
特異性
This antibody reacts weakly with Laminin alpha 3
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human, Recombinant fragment -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse Laminin alpha 5/LAMA5 and alpha 3 fragment recombinant proteins; Human fetal kidney lysate; A549 whole cell lysate; Mouse P5 fetal kidney and P3 fetal lung lysates; Rat kidney lysate.
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特記事項
ab271947 is the carrier-free version of ab184330.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18919 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271947の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 404 kDa (predicted molecular weight: 400 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 404 kDa (predicted molecular weight: 400 kDa). |
ターゲット情報
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機能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
組織特異性
Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver. -
配列類似性
Contains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
ドメイン
Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer. -
細胞内局在
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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参照データベース
- Entrez Gene: 3911 Human
- Entrez Gene: 16776 Mouse
- Entrez Gene: 140433 Rat
- Omim: 601033 Human
- SwissProt: O15230 Human
- SwissProt: Q61001 Mouse
- Unigene: 473256 Human
- Unigene: 4339 Mouse
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別名
- KIAA0533 antibody
- KIAA1907 antibody
- LAMA5 antibody
see all
画像
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All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 400 kDa
Observed band size: 404 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, sodium azide and BSA (ab184330).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab271947 は論文での使用が確認できていません。