Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - pH 9.0 Citrate Buffer Antigen Retrieval
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Rat Alexa Fluor
Host species: Rat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Rat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Staining works better if left incubating overnight at 4C. However, it does need several washes but the signal is strong and clear.
This is a section of wild type kidney tissue, paraffin embedded sample.
Anti-Laminin 2 alpha antibody GREEN
Dapi BLUE
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 07 2017