Application
Western blot
Sample
Mouse Tissue lysate - whole (intestinal mucosa)
Loading amount
70 µg
Specification
intestinal mucosa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2.5% Milk powder in PBS/Tween 0.3%
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG - HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 200 kDa Non-specific: 180, 60 kDa
Additional data
Additional Notes
Lanes on the blot:
1: control
2: 1h after manipulation
3: 3h after manipulation
4: 6h after manipulation
5: 12h after manipulation
6: 24h after manipulation
1: control
2: 1h after manipulation
3: 3h after manipulation
4: 6h after manipulation
5: 12h after manipulation
6: 24h after manipulation
Abcam response
TB 25/01/08 Mouse and WB tested.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sofia Tedelind
Verified customer
投稿 Feb 11 2008