Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231)
Specification
MDA-MB-231
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: block
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
PFA fixed tumor cells plated on collagen type IV in 8-well slides.
Micrograph demonstrates detection of laminin in breast tumor cells including punctate nuclear expression (green). Blue = hoechst.
Micrograph demonstrates detection of laminin in breast tumor cells including punctate nuclear expression (green). Blue = hoechst.
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投稿 May 06 2009