Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (normal brain)
Specification
normal brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
TNB Blocking Reagent (from another company) as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TNB
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/250
Additional data
Additional Notes
This is FoFr tissue (perfusion-fixed, no postfixation) and NOT FFPE. This antibody is reasonably potent and can be used with basic indirect immunofluorescent techniques in tissue with decent results. However, in the brain there is some background staining and faint staining of neuronal bodies. For laminin I typically use abcam's chicken polyclonal (ab14055) which is squeakly clean and potent. However, it reacts with more isoforms. Image demonstrates small vessels in the neocortex of a mouse (red).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 28 2008