Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Acetone
Permeabilization
No
Blocking step
TNB Blocking Reagent (from another company) as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TNB
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/250
Additional data
Additional Notes
This ab works well in tissue with standard indirect immunofluorescent techniques. However, it does have a share of background/nonspecific staining of neurons at higher titers. Personally, I find the chicken polyclonal anti-laminin offered by abcam to be vastly superior and very clean. Image demonstrates low power of human neocortex with laminin in vascular basement membranes (red).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 28 2008