Anti-Lamin B2 抗体 [EPR9701(B)] (ab151735)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9701(B)] to Lamin B2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Lamin B2 antibody [EPR9701(B)]
Lamin B2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9701(B)] to Lamin B2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Lamin B2 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q03252 -
ポジティブ・コントロール
- IP: HepG2 cell lysate; IHC: Human bladder cancer tissue; Mouse and rat testis; mouse kidney and cerebrum. ICC/IF: HepG2 cells; WB: HepG2 and HeLa cells, Mouse and rat testis and rat spleen lysates. Flow Cyt (intra): HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9701(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151735の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/20.
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WB |
1/2000. Predicted molecular weight: 68 kDa.
For unpurified use at 1/1000 - 1/10000. |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The human and rat recommendation is based on the WB results. We do not guarantee IHC-P for human and rat. |
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IP |
1/10 - 1/100.
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ICC/IF | (2) |
1/50.
For unpurified use at 1/250 - 1/500. |
特記事項 |
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Flow Cyt (Intra)
1/20. |
WB
1/2000. Predicted molecular weight: 68 kDa. For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The human and rat recommendation is based on the WB results. We do not guarantee IHC-P for human and rat. |
IP
1/10 - 1/100. |
ICC/IF
1/50. For unpurified use at 1/250 - 1/500. |
ターゲット情報
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機能
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
関連疾患
Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
細胞内局在
Nucleus inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 84823 Human
- Entrez Gene: 16907 Mouse
- Entrez Gene: 299625 Rat
- Omim: 150341 Human
- SwissProt: Q03252 Human
- SwissProt: P21619 Mouse
- Unigene: 728836 Human
- Unigene: 7362 Mouse
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別名
- LAMB 2 antibody
- LAMB2 antibody
- Lamin-B2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissues labelling Lamin B2 with ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on rat testis. The section was incubated with ab151735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissues labelling Lamin B2 with ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse testis. The section was incubated with ab151735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissues labelling Lamin B2 with ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse kidney. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissues labelling Lamin B2 with ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse cerebrum. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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All lanes : Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse testis lysate
Lane 3 : Rat spleen lysate
Lane 4 : Rat testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking Buffer and concentration: 5% NFDM/TBST
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Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking Buffer and concentration: 5% NFDM/TBST
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Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab151735 at 1:50 dilution (2.18 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab151735 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder cancer tissue sections labeling Lamin B2 with Purified ab151735 at 1:100 dilution (1.09 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Purified ab151735 at 1/20 dilution (0.5µg) immunoprecipitating Lamin B2 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab151735 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151735 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 68 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab151735 は 33 報の論文で使用されています。
- Jiang H et al. Sulfiredoxin-1 Inhibits PDGF-BB-Induced Vascular Smooth Muscle Cell Proliferation and Migration by Enhancing the Activation of Nrf2/ARE Signaling. Int Heart J 63:113-121 (2022). PubMed: 35034915
- Nguyen LTH et al. Coptisine Alleviates Imiquimod-Induced Psoriasis-like Skin Lesions and Anxiety-like Behavior in Mice. Molecules 27:N/A (2022). PubMed: 35209199
- Thi Huong Nguyen L et al. Seungmagalgeun-Tang, a Traditional Herbal Formula, Alleviates Skin Inflammation and Depression-Like Behavior in Atopic Dermatitis Mice under Sleep Deprivation Conditions. Evid Based Complement Alternat Med 2022:1307173 (2022). PubMed: 35368752
- Kono Y et al. Nucleoplasmic lamin C rapidly accumulates at sites of nuclear envelope rupture with BAF and cGAS. J Cell Biol 221:N/A (2022). IHC-P ; Human . PubMed: 36301259
- Gil L et al. Pathological Nuclear Hallmarks in Dentate Granule Cells of Alzheimer's Patients: A Biphasic Regulation of Neurogenesis. Int J Mol Sci 23:N/A (2022). PubMed: 36361662