Application
Western blot
Sample
Human Cell lysate - whole cell (cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
60 µg
Specification
cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Concentration
1 µg/ml
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
6 second(s)
Bands
Specific: 68 kDa Non-specific: 30, 40, 140 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 22 2021