Application
Western blot
Sample
Rat Tissue lysate - nuclear (soleus muscle)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10)
Loading amount
10 µg
Specification
soleus muscle
Blocking step
No blocking step used for 12 hour(s) and 0 minute(s) · Concentration: 1/1000µg/mL · Temperature: 4°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Inna Paramonova
Verified customer
投稿 Sep 26 2019