Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Intestine, spleen, liver.)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9
Permeabilization
No
Specification
Intestine, spleen, liver.
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 25°C · Diluent: TRIS based diluent
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Vector Lab ImmPRESS Anti-rabbit polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Formalin fixation was kept under 7 days. Retrieval was at 95C for 20 minutes. Enzymatic retrieval did not work. No retrieval showed promise and may be worked out. Heat retrieval with several buffers worked best.
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in pig, a species that has not been tested before. We welcome any further feedback and AbReviews from other researchers who have used this antibody in pig which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Victor Bernal
Verified customer
投稿 Apr 23 2018