Anti-Lambda Free Light Chain 抗体 [3D12] (ab1944)

Reviews (1)Q&A (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)

    Key features and details

    • Mouse monoclonal [3D12] to Lambda Free Light Chain
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-Lambda Free Light Chain antibody [3D12]

    • 製品の詳細

      Mouse monoclonal [3D12] to Lambda Free Light Chain

    • 由来種

      Mouse

    • 特異性

      Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Mixture of light chains of human immunoglobulin.

    • ポジティブ･コントロール

      • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

    • 特記事項

      Concentration varies from lot to lot and can be provided on request.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • バッファー

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 精製度

      Protein A purified

    • 特記事項（精製）

      Purity tested by electrophoresis.

    • ポリ/モノ

      モノクローナル

    • クローン名

      3D12

    • ミエローマ

      Sp2/0

    • アイソタイプ

      IgG2a

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab1944の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 5 µg/ml.
    特記事項
    IHC-P
    Use a concentration of 5 µg/ml.

    ターゲット情報

    • 関連性

      standard

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)

      IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献 (0)

    ab1944 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1944 は論文での使用が確認できていません。

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    Flow Cytometry abreview for Anti-Lambda Free Light Chain antibody [3D12]

     Inconclusive
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Pig Cell (blood cells)
    Permeabilization
    No
    Gating Strategy
    Lymphoid gate only
    Specification
    blood cells
    Preparation
    Cell harvesting/tissue preparation method: hypotonic lysis of erythrocytes
    Sample buffer: phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin from Cold Water Fish Skin
    Fixation
    None
    Read More

    Dr. Marek Sinkora

    Verified customer

    投稿 Jun 20 2016

    Question

    How were these antibodies tested in Western blot (full protocol)? How should plasma samples be used in Western blot with these antibodies?

    Read More
    Answer

    Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry.

    The protocolused for Western blotting was according to Towbin et al. in: PNAS, September 1, 1979, vol. 76, no. 9, 4350-4354: Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications. Please find this article attached to this email.


    The lab has not done any testing on these antibodies with plasma samples, so unfortunately we do not have an in-house protocol for this sample type. The positive control used was Bence-Jones protein separated from the urine of a multiple myeloma patient.
    I've looked through some literature and found some resources that might be helpful for running plasma samples on a Western blot-
    http://qjmed.oxfordjournals.org/content/90/3/197.full.pdf
    http://vir.sgmjournals.org/content/69/3/723.full.pdf
    http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0008701
    I would recommend treating the plasma samples as you would treat a lysate, by measuring protein concentration and diluting in sample buffer. The optimal protein loading will need to be determined intrinsically for your particular samples, but the above references should give some idea of reasonable protein loading range.
    I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you, and I'll be happy to help.

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