製品の概要
-
製品名Anti-Lambda Free Light Chain antibody [3D12]
-
製品の詳細Mouse monoclonal [3D12] to Lambda Free Light Chain
-
由来種Mouse
-
特異性Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.
-
アプリケーション適用あり: WB, ELISA, Other, IHC-Pmore details
-
種交差性交差種: Human
-
免疫原
Mixture of light chains of human immunoglobulin.
-
ポジティブ･コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
-
特記事項Concentration varies from lot to lot and can be provided on request.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
バッファーPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
精製度Protein A purified
-
特記事項（精製）Purity tested by electrophoresis.
-
ポリ/モノモノクローナル
-
クローン名3D12
-
ミエローマSp2/0
-
アイソタイプIgG2a
-
研究分野
関連製品
-
Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab1944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| AP | Use at an assay dependent concentration. | |
| Other | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 5 µg/ml. |
ターゲット情報
-
関連性standard
画像
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)](https://www.abcam.co.jp/ps/datasheet/images/1/ab1944/Lambda-Free-Light-Chain-Primary-antibodies-ab1944-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
参考文献
ab1944 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"