Anti-Lambda Free Light Chain 抗体 [3D12] (ab1944)

Reviews (1)Q&A (1)

製品の概要

  • 製品名

    Anti-Lambda Free Light Chain antibody [3D12]

  • 製品の詳細

    Mouse monoclonal [3D12] to Lambda Free Light Chain

  • 由来種

    Mouse

  • 特異性

    Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.

  • アプリケーション

    適用あり: WB, ELISA, Other, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Mixture of light chains of human immunoglobulin.

  • ポジティブ･コントロール

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

  • 特記事項

    Concentration varies from lot to lot and can be provided on request.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Purity tested by electrophoresis.

  • ポリ/モノ

    モノクローナル

  • クローン名

    3D12

  • ミエローマ

    Sp2/0

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 関連性

    standard

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)

    IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

ab1944 has not yet been referenced specifically in any publications.

ab1944 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Flow Cytometry abreview for Anti-Lambda Free Light Chain antibody [3D12]

 Inconclusive
Abreviews
abreview image
Application
Flow Cytometry
Sample
Pig Cell (blood cells)
Permeabilization
No
Gating Strategy
Lymphoid gate only
Specification
blood cells
Preparation
Cell harvesting/tissue preparation method: hypotonic lysis of erythrocytes
Sample buffer: phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin from Cold Water Fish Skin
Fixation
None
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Dr. Marek Sinkora

Verified customer

投稿 Jun 20 2016

Question

How were these antibodies tested in Western blot (full protocol)? How should plasma samples be used in Western blot with these antibodies?

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Answer

Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry.

The protocolused for Western blotting was according to Towbin et al. in: PNAS, September 1, 1979, vol. 76, no. 9, 4350-4354: Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications. Please find this article attached to this email.


The lab has not done any testing on these antibodies with plasma samples, so unfortunately we do not have an in-house protocol for this sample type. The positive control used was Bence-Jones protein separated from the urine of a multiple myeloma patient.
I've looked through some literature and found some resources that might be helpful for running plasma samples on a Western blot-
http://qjmed.oxfordjournals.org/content/90/3/197.full.pdf
http://vir.sgmjournals.org/content/69/3/723.full.pdf
http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0008701
I would recommend treating the plasma samples as you would treat a lysate, by measuring protein concentration and diluting in sample buffer. The optimal protein loading will need to be determined intrinsically for your particular samples, but the above references should give some idea of reasonable protein loading range.
I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you, and I'll be happy to help.

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