Anti-LAMA1 抗体 [CL3087] (ab210954)
Key features and details
- Mouse monoclonal [CL3087] to LAMA1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-LAMA1 antibody [CL3087]
LAMA1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CL3087] to LAMA1 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human LAMA aa 600-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P25391 -
エピトープ
ab210954 binds to an epitope located within the peptide sequence LVPENFQDFHSKRQI (aa 640 - 654) -
ポジティブ・コントロール
- WB: HeLa cell lysate; Human testis tissue lysate. IHC-P: Human lymph node and testis tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
CL3087 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab210954の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Predicted molecular weight: 337 kDa.
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特記事項 |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 337 kDa. |
ターゲット情報
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機能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
関連疾患
Poretti-Boltshauser syndrome -
配列類似性
Contains 17 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 2 laminin IV type A domains.
Contains 1 laminin N-terminal domain. -
ドメイン
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI, IV and G are globular. -
翻訳後修飾
Tyrosine phosphorylated by PKDCC/VLK. -
細胞内局在
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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参照データベース
- Entrez Gene: 284217 Human
- Entrez Gene: 16772 Mouse
- Entrez Gene: 316758 Rat
- Omim: 150320 Human
- SwissProt: P25391 Human
- SwissProt: P19137 Mouse
- Unigene: 270364 Human
- Unigene: 303386 Mouse
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別名
- LAMA antibody
- LAMA1 antibody
- LAMA1_HUMAN antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling LAMA1 using ab210954 at a 1/200 dilution.
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All lanes : Anti-LAMA1 antibody [CL3087] (ab210954) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LAMA1 knockout HeLa cell lysate
Lane 3 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 337 kDa
Observed band size: 337 kDaLanes 1-3: Merged signal (red and green). Green - ab210954 observed at 337 kDa. Red - loading control ab129002 observed at 124 kDa.
ab210954 Anti-LAMA1 antibody [CL3087] was shown to specifically react with LAMA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264991 (knockout cell lysate ab258937) was used. Wild-type and LAMA1 knockout samples were subjected to SDS-PAGE. ab210954 and Anti-Vinculin antibody [EPR8185] (ab129002) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human lymph node tissue (negative control), labeling LAMA1 using ab210954 at a 1/200 dilution.
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All lanes : Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution
Lane 1 : Recombinant protein fragment of human Laminin 111
Lane 2 : Recombinant protein fragment of human Laminin 121
Lane 3 : Recombinant protein fragment of human Laminin 221
Lane 4 : Recombinant protein fragment of human Laminin 332
Lane 5 : Recombinant protein fragment of human Laminin 411
Lane 6 : Recombinant protein fragment of human Laminin 521
Developed using the ECL technique.
Predicted band size: 337 kDa -
Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution + Human testis tissue lysate
Developed using the ECL technique.
Predicted band size: 337 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab210954 は論文での使用が確認できていません。