Anti-LAIR1 抗体 [113] - Low endotoxin, Azide free (ab171239)
Key features and details
- Armenian hamster monoclonal [113] to LAIR1 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-LAIR1 antibody [113] - Low endotoxin, Azide free
LAIR1 一次抗体 製品一覧 -
製品の詳細
Armenian hamster monoclonal [113] to LAIR1 - Low endotoxin, Azide free -
由来種
Armenian hamster -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
The details of the immunogen for this antibody are not available.
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ポジティブ・コントロール
- Mouse splenocytes.
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特記事項
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
113 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab171239の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use at an assay dependent concentration.
ab171239 can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. ab171239 can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells. -
組織特異性
Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
発生段階
Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation. -
ドメイン
ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation. -
翻訳後修飾
Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
N-glycosylated. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 52855 Mouse
- SwissProt: Q8BG84 Mouse
- Unigene: 290880 Mouse
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別名
- 5133400O11Rik antibody
- CD305 antibody
- D7Bwg0421e antibody
see all
画像
データシートおよび資料
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Datasheet download
参考文献 (1)
ab171239 は 1 報の論文で使用されています。
- Kang X et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . PubMed: 25915125