Anti-LAG-3 抗体 [BLR028F] - BSA free (ab243885)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR028F] to LAG-3 - BSA free
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
製品の概要
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製品名
Anti-LAG-3 antibody [BLR028F] - BSA free
LAG-3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [BLR028F] to LAG-3 - BSA free -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC, IHC-P, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human LAG-3 (extracellular).
Database link: P18627 -
ポジティブ・コントロール
- WB: HDLM-2 whole cell lysate. IP: HDLM-2 whole cell lysate. ICC: HDLM-2 cells. IHC-P: Human tonsil tissue.
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特記事項
This product is sold under License from Bethyl Laboratories, Inc.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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特記事項(精製)
Recombinant antibody was purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
BLR028F -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab243885の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000.
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ICC |
1/100 - 1/500.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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|
IP |
Use at an assay dependent concentration.
Use 6µl/0.5mg lysate. |
特記事項 |
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WB
1/1000. |
ICC
1/100 - 1/500. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. Use 6µl/0.5mg lysate. |
ターゲット情報
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機能
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
組織特異性
On cell surface of activated NK and T-lymphocytes. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
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別名
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
see all
画像
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Western blot analysis using ab243885 at 1/1000 dilution.
Lane 1: HDLM-2 whole cell lysate (5 µg).
Lane 2: RPMI 8226 whole cell lysate (5 µg).
Lane3: Jurkat whole cell lysate (5 µg).
Lane 4: MJ whole cell lysate (5 µg).
Lane 5: HEK-293T whole cell lysate (5 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
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LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243885 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243885 at 1/1000 dilution.
Lane 1: ab243885 IP in HDLM-2 whole cell lysate.
Lane 2: Control IgG IP in HDLM-2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243885 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243885 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 (red) using ab243885 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DABI staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab243885 は論文での使用が確認できていません。