Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8502] to LACTB - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
製品名Anti-LACTB antibody [EPR8502] - BSA and Azide free
LACTB 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR8502] to LACTB - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human LACTB aa 100-200. The exact sequence is proprietary.
Database link: P83111
Ab248369 is the carrier-free version of ab131171. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248369 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 1.27 x 10 -10 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).|
関連性LACTB is a protein from the large 39S subunit of the mitochondrial ribosome (mitoribosome). It has some sequence similarity to prokaryotic beta-lactamases but most of the residues that are responsible for the beta-lactamase activity are not conserved between the two proteins. It is expressed predominantly in skeletal muscle. There are two named isoforms.
- FLJ14902 antibody
- G24 antibody
- Lactamase, beta antibody
All lanes : Anti-LACTB antibody [EPR8502] (ab131171) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart cell lysate
Lane 4 : Human fetal muscle lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
This data was developed using ab131171, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.