Anti-Lactate Dehydrogenase LDHA + LDHB 抗体 [10E6AA9] (ab112996)
Key features and details
- Mouse monoclonal [10E6AA9] to LDHA + LDHB
- Suitable for: ICC/IF, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] -
製品の詳細
Mouse monoclonal [10E6AA9] to LDHA + LDHB -
由来種
Mouse -
特異性
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques. -
アプリケーション
適用あり: ICC/IF, IP, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- HeLa cells and cell lysate.
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading...
-
精製度
Proprietary Purification -
特記事項(精製)
ab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
ポリ/モノ
モノクローナル -
クローン名
10E6AA9 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112996の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 2 µg/ml.
|
|
IP |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
Triton permeabilization required. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
---|
ICC/IF
Use a concentration of 2 µg/ml. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use a concentration of 1 - 2 µg/ml. Triton permeabilization required. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
画像
-
ab112996 at 2ug/ml staining Lactate Dehydrogenase B/LDH-B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
-
ab112996 at 1ug/ml staining Lactate Dehydrogenase B/LDH-B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
-
The 36.6 kDa LDHB protein along with 36.7kDa LDHA protein were immunoprecipitated from Hela cell lysate with ab112996. The identities of the proteins were confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab112996 は 1 報の論文で使用されています。
- Ran F et al. miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis. J Oncol 2021:5529770 (2021). PubMed: 33986801