遺伝子ノックアウト細胞株 検証済
リコンビナント
RabMAb

Anti-Lactate Dehydrogenase B / LDH-B 抗体 [EP1565Y] (ab53292)

製品の概要

  • 製品名
    Anti-Lactate Dehydrogenase B / LDH-B antibody [EP1565Y]
    Lactate Dehydrogenase B / LDH-B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B / LDH-B
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-Fr, WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human Lactate Dehydrogenase B/ LDH-B aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P07195

  • ポジティブ・コントロール
    • HeLa cell lysate Human heart muscle
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Lactate Dehydrogenase B.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
WB 1/50000 - 1/200000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP 1/100.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.

ターゲット情報

  • パスウェイ
    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • 関連疾患
    Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
  • 配列類似性
    Belongs to the LDH/MDH superfamily. LDH family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epididymis secretory protein Li 281 antibody
    • HEL S 281 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase B chain antibody
    • Lactate Dehydrogenase B antibody
    • Lactate dehydrogenase H chain antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH-B antibody
    • LDH-H antibody
    • LDHB antibody
    • LDHB_HUMAN antibody
    • LDHBD antibody
    • LDHH antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-46 antibody
    • TRG-5 antibody
    • TRG5 antibody
    see all

画像

  • All lanes : Anti-Lactate Dehydrogenase B / LDH-B antibody [EP1565Y] (ab53292) at 1/50000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : LDHB (Lactate Dehydrogenase B) knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 37 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab53292 was shown to specifically react with Lactate Dehydrogenase B in wild-type HAP1 cells as signal was lost in LDHB (Lactate Dehydrogenase B) knockout cells. Wild-type and LDHB (Lactate Dehydrogenase B) knockout samples were subjected to SDS-PAGE. Ab53292 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • ICC/IF image of ab53292 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53292, neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Human heart muscle labeled with ab53292 at 1/100 - 1/250 dilution

  • Anti-Lactate Dehydrogenase B / LDH-B antibody [EP1565Y] (ab53292) + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • Overlay histogram showing HeLa cells stained with ab53292 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53292, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Berrios C  et al. Merkel Cell Polyomavirus Small T Antigen Promotes Pro-Glycolytic Metabolic Perturbations Required for Transformation. PLoS Pathog 12:e1006020 (2016). WB ; Human . Read more (PubMed: 27880818) »
  • Vaughan RA  et al. ß-alanine suppresses malignant breast epithelial cell aggressiveness through alterations in metabolism and cellular acidity in vitro. Mol Cancer 13:14 (2014). WB ; Human . Read more (PubMed: 24460609) »
See all 7 Publications for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer pH 9.0
Permeabilization
Yes - Wash Buffer from Dako with Tween
Specification
Tonsil
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

投稿 Jul 19 2018

Answer

Vielen Dank für Ihre Anfrage.

Es handelt sich hierbei um das selbe Epitomics- Produkt, da wir unsere Produktpalette mit diesen Antikörpern erweitert haben, und diese Antikörper wurden 1:1 aus dem Epitomics Repertoire übernommen, und auch noch im Epitomics Labor hergestellt.

Das es sich hierbei um den selben Antikörper handelt können sie übrigens auch an der Klon-Nummer erkennen. Da auch das Immunogen das gleiche ist, sollte die Spezifität mit der Isoform B gegeben sein. Und in der Tat kann man diesen Hinweis auf unserem Datenblatt finden:



A synthetic peptide corresponding to residues near the N-terminus of human LDH-B.


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録