Anti-Lactate Dehydrogenase B/LDH-B 抗体 (ab195964)
Key features and details
- Goat polyclonal to Lactate Dehydrogenase B/LDH-B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Lactate Dehydrogenase B/LDH-B antibody
Lactate Dehydrogenase B/LDH-B 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Lactate Dehydrogenase B/LDH-B -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Guinea pig, Chimpanzee, Monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Lactate Dehydrogenase B/LDH-B aa 1-100 (internal sequence) (Cysteine residue). (Near N-terminus; NP_002291.1).
Database link: P07195 -
ポジティブ・コントロール
- Human heart and kidney lysates; Human kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab195964の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
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IHC-P |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa). |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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パスウェイ
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
関連疾患
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease. -
配列類似性
Belongs to the LDH/MDH superfamily. LDH family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 465337 Chimpanzee
- Entrez Gene: 100033997 Horse
- Entrez Gene: 3945 Human
- Omim: 150100 Human
- SwissProt: A5A6N7 Chimpanzee
- SwissProt: P07195 Human
- Unigene: 446149 Human
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別名
- Epididymis secretory protein Li 281 antibody
- HEL S 281 antibody
- L lactate dehydrogenase B chain antibody
see all
画像
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All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody (ab195964) at 0.3 µg/ml
Lane 1 : Human heart lysate (in RIPA buffer)
Lane 2 : Human kidney lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
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Immunohistochemical anaysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Lactate Dehydrogenase B/LDH-B with ab195964 at 5 µg/mL.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab195964 は論文での使用が確認できていません。