Key features and details
- Mouse monoclonal [9A5] to LacI
- Suitable for: WB, ICC/IF, IHC-FoFr
- Reacts with: Escherichia coli
- Isotype: IgG1
製品名Anti-LacI antibody [9A5]
製品の詳細Mouse monoclonal [9A5] to LacI
特異性The clone number has been updated from (0.T.81) to (9A5) both clone numbers name the same antibody clone.
アプリケーション適用あり: WB, ICC/IF, IHC-FoFrmore details
種交差性交差種: Escherichia coli
Recombinant TrpE fusion protein containing full length E. coli LacI.
- Lac1 transfected Cos-1 cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.05% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
精製度Protein G purified
Our Abpromise guarantee covers the use of ab33832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fo: Use at an assay dependent dilution. The antibody shows nuclear immunostaining in Lac1 transgenic tissue fixed with 4% paraformaldehyde and permeabilized with Triton X100.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- DNA binding transcriptional repressor antibody
- LacI antibody
- Transcriptional repressor of the lac operon antibody
Ab33832 (1/1000) staining Lacl in Escherichia coli cultured cell lysate
Secondary Antibody: An HRP-conjugated Donkey anti-mouse monoclonal (1/20000)
Lane 1: Molecular weight Marker
Lane 2: Rainbow Molecular weight Marker
Lane 3: Blank
Lane 4: pCMV Vector cell lysate 1
Lane 5: pCMV Vector cell lysate 2
Performed under reduced and denaturing running conditions.
Blocking step: 5% Milk for 1 hour at 22°C.
ab33832 は 6 報の論文で使用されています。
- Malherbe G et al. A robust fractionation method for protein subcellular localization studies in Escherichia coli. Biotechniques 66:171-178 (2019). PubMed: 30987443
- Russell CW & Mulvey MA The Extraintestinal Pathogenic Escherichia coli Factor RqlI Constrains the Genotoxic Effects of the RecQ-Like Helicase RqlH. PLoS Pathog 11:e1005317 (2015). PubMed: 26636713
- Sienski G et al. Silencio/CG9754 connects the Piwi-piRNA complex to the cellular heterochromatin machinery. Genes Dev 29:2258-71 (2015). Human . PubMed: 26494711
- Du P et al. Methyltransferase-like 17 physically and functionally interacts with estrogen receptors. IUBMB Life 67:861-8 (2015). PubMed: 26488768
- Kogenaru M & Tans SJ An improved Escherichia coli strain to host gene regulatory networks involving both the AraC and LacI inducible transcription factors. J Biol Eng 8:2 (2014). WB . PubMed: 24382032
- Purcell O et al. Temperature dependence of ssrA-tag mediated protein degradation. J Biol Eng 6:10 (2012). WB . PubMed: 22824000