製品の概要
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製品名Anti-Ku70 antibody
Ku70 一次抗体 製品一覧 -
製品の詳細Mouse polyclonal to Ku70
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由来種Mouse
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
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免疫原
Recombinant protein encoding a C-terminal fragment of human Ku 70 expressed in E.coli.
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ポジティブ･コントロール
- HeLa or Raji whole cell or nuclear lysate. Recombinant human Ku 70 can also be used.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab2624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 70 kDa). |
ターゲット情報
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機能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
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配列類似性Belongs to the ku70 family.
Contains 1 Ku domain.
Contains 1 SAP domain. -
発生段階Expression does not increase during promyelocyte differentiation.
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翻訳後修飾Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
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細胞内局在Nucleus. Chromosome.
- Information by UniProt
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参照データベース
- Entrez Gene: 2547 Human
- Omim: 152690 Human
- SwissProt: P12956 Human
- Unigene: 292493 Human
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別名
- 5''-deoxyribose-5-phosphate lyase Ku70 antibody
- 5''-dRP lyase Ku70 antibody
- 70 kDa subunit of Ku antigen antibody
see all
画像
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Western blot - Anti-Ku70 antibody (ab2624)ab2624 at a 1:2000 dilution staining Ku 70 in A) HeLa whole cell lysate and B) Raji whole cell lysate by Western Blot (ECL) ab2624 at a 1:2000 dilution staining Ku 70 in A) HeLa whole cell lysate and B) Raji whole cell lysate by Western Blot (ECL)
プロトコール
参考文献
ab2624 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"