Anti-KRT81 抗体 [3B10-5B10] (ab55407)
Key features and details
- Mouse monoclonal [3B10-5B10] to KRT81
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2b
製品の概要
-
製品名
Anti-KRT81 antibody [3B10-5B10]
KRT81 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3B10-5B10] to KRT81 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein, corresponding to amino acids 304-505 of Human KRT81.
-
特記事項
This product was changed from ascites to tissue culture supernatant on 27/June/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4 -
Concentration information loading...
-
精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
3B10-5B10 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab55407の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | ||
ICC/IF | ||
IHC-P |
特記事項 |
---|
IF/ICC: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
-
組織特異性
Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla. Expressed in malignant lymph node tissue in breast carcinoma tissue. -
関連疾患
Defects in KRT81 are a cause of monilethrix (MLTRX) [MIM:158000]. Monilethrix is an autosomal dominant hair disorder characterized clinically by alopecia and follicular papules. Affected hairs have uniform elliptical nodes of normal thickness and intermittent constrictions, internodes at which the hair easily breaks. Usually only the scalp is involved, but in severe forms, the secondary sexual hair, eyebrows, eyelashes, and nails may also be affected. -
配列類似性
Belongs to the intermediate filament family. - Information by UniProt
-
参照データベース
- Entrez Gene: 3887 Human
- Omim: 602153 Human
- SwissProt: Q14533 Human
- Unigene: 658118 Human
-
別名
- 1 antibody
- basic antibody
- ghHb 1 antibody
see all
画像
-
KRT81 antibody (ab55407) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human esophagus. This was producted using the ascites version of the product.
-
KRT81 antibody (ab55407) used in immunofluorescence at 10ug/ml on HeLa cells. This was producted using the ascites version of the product.
-
KRT81 antibody (ab55407) at 1ug/lane + HepG2 cell lysate at 25ug/lane. This was producted using the ascites version of the product.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab55407 は 2 報の論文で使用されています。
- Wang X et al. Single-cell RNA-seq reveals the genesis and heterogeneity of tumor microenvironment in pancreatic undifferentiated carcinoma with osteoclast-like giant-cells. Mol Cancer 21:133 (2022). PubMed: 35733218
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873