Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA-MB-231 tumor taken from Balb/C nude mice)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
MDA-MB-231 tumor taken from Balb/C nude mice
Blocking step
Innovex background buster as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SignalStain® Antibody Diluent #8112
Dilution
1/75
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Flu
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Green: K-ras,
Blue: DAPI
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 22 2018