Application
Western blot
Sample
Human Cell lysate - nuclear (SKBR3, MDA231, U937, THP1, OV90, HCT116 MDA468, AM)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
SKBR3, MDA231, U937, THP1, OV90, HCT116 MDA468, AM
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody #7074
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL with licor c-digit
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 21 kDa Non-specific: 110, 80, 70, 60, 40, 45, 1 kDa
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Abcam user community
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投稿 May 22 2018