Anti-Ras 抗体 (ab180772)
Key features and details
- Rabbit polyclonal to Ras
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Ras antibody
Ras 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ras -
由来種
Rabbit -
特異性
The immunogen used for this product shares 82% homology with HRAS and NRAS. Cross-reactivity with these proteins has not been confirmed experimentally.
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アプリケーション
適用あり: IP, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human Ras aa 30-189.
Sequence:D EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCIIM
Database link: P01116 -
ポジティブ・コントロール
- WB: A-549, HeLa and Raji cell lysates. Mouse spleen, lung and rat brain tissue extracts. ICC/IF: C6 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180772の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 21 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF | (1) |
1/50 - 1/200.
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 21 kDa. |
IHC-P
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/50 - 1/200. |
ターゲット情報
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機能
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. -
関連疾患
Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC). -
配列類似性
Belongs to the small GTPase superfamily. Ras family. -
翻訳後修飾
Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. -
細胞内局在
Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 3265 Human
- Entrez Gene: 3845 Human
- Entrez Gene: 4893 Human
- Entrez Gene: 15461 Mouse
- Entrez Gene: 16653 Mouse
- Entrez Gene: 18176 Mouse
- Entrez Gene: 24525 Rat
- Entrez Gene: 24605 Rat
see all -
別名
- C BAS/HAS antibody
- C HA RAS1 antibody
- C-BAS/HAS antibody
see all
画像
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Immunofluorescence staining of C6 cells stained for RAS with ab180772 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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All lanes : Anti-Ras antibody (ab180772) at 1/1000 dilution
Lane 1 : A-549 cell extract
Lane 2 : HeLa cell extract
Lane 3 : Raji cell extract
Lane 4 : Mouse spleen tissue extract
Lane 5 : Mouse lung tissue extract
Lane 6 : Rat brain tissue extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 21 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab180772 は 33 報の論文で使用されています。
- Zhang Y et al. LINC01354/microRNA-216b/KRAS Axis Promotes the Occurrence and Metastasis of Endometrial Cancer. Nanoscale Res Lett 17:21 (2022). PubMed: 35099637
- Del Gaudio N et al. CBX2 shapes chromatin accessibility promoting AML via p38 MAPK signaling pathway. Mol Cancer 21:125 (2022). PubMed: 35681235
- Yang Y et al. KRAS, YWHAE, SP1 and MSRA as biomarkers in endometrial cancer. Transl Cancer Res 10:1295-1312 (2021). PubMed: 35116456
- Wang Y et al. Positive Crosstalk Between Hedgehog and NF-?B Pathways Is Dependent on KRAS Mutation in Pancreatic Ductal Adenocarcinoma. Front Oncol 11:652283 (2021). PubMed: 34046348
- Wu N et al. KRAS gene status in gastric signet-ring cell carcinoma patients and acts as biomarker of MEK inhibitor. J Gastrointest Oncol 12:1020-1030 (2021). PubMed: 34295553