アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報

新型コロナウイルス (COVID-19)に伴う当社の対応について

  1. Link

    kpnb1-antibody-3e9-ab2811.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Nucleus Nuclear Envelope
Share by email

Anti-KPNB1 抗体 [3E9] (ab2811)

  • Datasheet
  • SDS
Reviews (19)Q&A (3)References (48)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
  • Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [3E9] (ab2811)
  • Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [3E9] (ab2811)
  • Immunoprecipitation - Anti-KPNB1 antibody [3E9] (ab2811)
  • Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)
  • Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
  • Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
  • Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
  • Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [3E9] (ab2811)
  • Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)
  • Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)
  • Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)

Key features and details

  • Mouse monoclonal [3E9] to KPNB1
  • Suitable for: Flow Cyt, IP, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

こちらの製品もご検討ください

アッセイ
Product image
F-actin Staining Kit - Blue Fluorescence - Cytopainter (ab112124)
一次抗体
Product image
Anti-Ran antibody [EPR10791(B)] (ab155103)
一次抗体
Product image
Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413)

関連製品

製品の概要

  • 製品名

    Anti-KPNB1 antibody [3E9]
    KPNB1 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [3E9] to KPNB1
  • 由来種

    Mouse
  • アプリケーション

    適用あり: Flow Cyt, IP, WB, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow KPNB1. Purified from Bovine erythrocytes.

  • ポジティブ・コントロール

    • WB: HeLa, Caco-2, NIH/3T3, U251 MG, HepG2 and C6 whole cell lysates. ICC: HMVEC cells, PTK cells. Flow Cyt: PC-12, Jurkat and NIH 3T3 cells.
  • 特記事項

    Previously labelled as NTF97/Importin beta. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • 一次抗体 備考

    The accumulation of proteins in the nucleus is mediated by short sequences of basic amino acids called nuclear localization sequences (NLSs). These sequences are necessary and sufficient to direct a protein or inert carrier to the nuclear interior. Nuclear protein import is accomplished by two sequential, energy dependent events. The first step, docking at the nuclear pore complex, requires a 54/56 kDa nuclear localization signal receptor (a-karyopherin, importin-a, SRP-a) and the nuclear transport factor p97 (NTF 97, b-karyopherin, importin-b). The second step, translocation across the nuclear envelope (NE), requires the GTP-binding protein, Ran/TC4.
  • ポリ/モノ

    モノクローナル
  • クローン名

    3E9
  • アイソタイプ

    IgG2a
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

関連製品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2811の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
WB (16)
1/5000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ICC/IF (3)
1/1000.
特記事項
Flow Cyt
1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
WB
1/5000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ICC/IF
1/1000.

ターゲット情報

  • 機能

    Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.
  • 配列類似性

    Belongs to the importin beta family.
    Contains 8 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • 細胞内局在

    Cytoplasm. Nucleus envelope.
  • Target information above from: UniProt accession Q14974 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3837 Human
    • Entrez Gene: 16211 Mouse
    • Entrez Gene: 24917 Rat
    • Omim: 602738 Human
    • SwissProt: Q14974 Human
    • SwissProt: P70168 Mouse
    • SwissProt: P52296 Rat
    • Unigene: 532793 Human
    • Unigene: 706168 Human
    • Unigene: 251013 Mouse
    • Unigene: 11061 Rat
    see all
  • 別名

    • IMB 1 antibody
    • IMB1 antibody
    • IMB1_HUMAN antibody
    • Impnb antibody
    • Importin 90 antibody
    • Importin beta 1 antibody
    • Importin beta 1 subunit antibody
    • Importin subunit beta-1 antibody
    • Importin-90 antibody
    • IPOB antibody
    • Karyopherin beta 1 antibody
    • Karyopherin beta 1 subunit antibody
    • Karyopherin subunit beta-1 antibody
    • KPNB 1 antibody
    • Kpnb1 antibody
    • MGC2155 antibody
    • MGC2156 antibody
    • MGC2157 antibody
    • NTF 97 antibody
    • NTF97 antibody
    • NTF97/Importin beta antibody
    • Nuclear factor P97 antibody
    • Pore targeting complex 97 kDa subunit antibody
    • PTAC97 antibody
    see all

画像

  • Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
    Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
    All lanes : Anti-KPNB1 antibody [3E9] (ab2811) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG H+L (HRP) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [3E9] (ab2811)
    Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [3E9] (ab2811)

    Immunofluorecent analysis of HMVEC (Human Lung Microvascular Endothelial cells) cells stained for KPNB1 using ab2811.

  • Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [3E9] (ab2811)
    Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [3E9] (ab2811)This image is courtesy of Roberto Giambruno, Marilena Ciciarello and Patrizia Lavia

    NIH/3T3 (Mouse embryo fibroblast cell line) cells were incubated for 4 minutes in PHEM/1%triton, washed for 2 minutes in 1x PHEM and fixed for 10 minutes at room temperature in 3.7% PFA containing 30mM sucrose. Following washing in PBS, the cells were incubated for 2 minutes in 100% Methanol at -20°C, then washed 3 times in PBS. The cells were then incubated with ab2811 (1/200) for 1 hour at room temperature. The image panel shows the nuclei stained with DAPI (blue) and the nuclear envalope and cytoplasm stained with ab2811 (green). 100x magnification.

     

     
     

     

     
     
  • Immunoprecipitation - Anti-KPNB1 antibody [3E9] (ab2811)
    Immunoprecipitation - Anti-KPNB1 antibody [3E9] (ab2811)Image and protocol courtesy of Rosamaria Mangiacasale and Patrizia Lavia, University 'La Sapienza' CNR, Italy

    Immunoprecipitation of Importin beta, in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, using ab2811. Coomassie-stained 8% SDS-page gel was loaded with IP fractions obtained by incubating 2 mg of pre-cleared HeLa whole cell extracts with 4µg ab2811 or 4µg IgG (control). The Importin band (see arrow) was cut out of the gel and its identity confirmed by Mass Spectometry. Please refer to protocol tab for further experimental details.

  • Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)
    Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)

    Flow cytometry analysis of KPNB1 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2811 (1ug/test) for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
    Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
    All lanes : Anti-KPNB1 antibody [3E9] (ab2811) at 1/50 dilution

    Lane 1 : U-251 MG (Human brain glioma cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP-conjugated secondary antibody

    Predicted band size: 97 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
    Western blot - Anti-KPNB1 antibody [3E9] (ab2811)This image is courtesy of an anonymous Abreview
    All lanes : Anti-KPNB1 antibody [3E9] (ab2811) at 1/2000 dilution

    Lane 1 : Mouse lung whole tissue lysate
    Lane 2 : Mouse kidney whole tissue lysate
    Lane 3 : Mouse spleen whole tissue lysate

    Secondary
    All lanes : HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 97 kDa


    Exposure time: 3 minutes

    See Abreview

  • Western blot - Anti-KPNB1 antibody [3E9] (ab2811)
    Western blot - Anti-KPNB1 antibody [3E9] (ab2811)Image and protocol courtesy of Rosamaria Mangiacasale and Patrizia Lavia, University 'La Sapienza' CNR, Italy
    Anti-KPNB1 antibody [3E9] (ab2811) at 1/5000 dilution + HeLa Whole cell extract

    Secondary
    HRP-conjugated anti mouse IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 97 kDa



    HeLa whole cell extract was run on a 10%SDS-PAGE and transferred to PVDF membrane. Membrane was blocked for 30 mins in TBS/0.1% Tween/ 5% Milk; ab2811 (1/5000) was incubated for 1 hr in TBS/0.1%Tween/5% Milk and followed by 3 washes in TBS/ 0.1%Tween (3x 7 mins). Secondary antibody was incubated for 30 mins in a TBS/ 0.1% Tween solution.This was followed by 3 washes with the TBST solution (3x7 mins) and one wash in TBS. Western blot developed using ECL plus (Amersham).
  • Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [3E9] (ab2811)
    Immunocytochemistry/ Immunofluorescence - Anti-KPNB1 antibody [3E9] (ab2811)

    Immunolocalization of KPNB1 in PTK (Long-nosed potoroo epithelial kidney cell line) cells using ab2811.

  • Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)
    Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)

    Flow cytometry analysis of KPNB1 in PC-12 (Rat adrenal gland pheochromocytoma cell line) cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2811 (1ug/test) for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)
    Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)

    Flow cytometry analysis of KPNB1 in 3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2811 (1ug/test) for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)
    Flow Cytometry - Anti-KPNB1 antibody [3E9] (ab2811)
    Overlay histogram showing Jurkat cells stained with ab2811 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2811, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

プロトコール

  • Immunoprecipitation protocol for ab2811

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (48)

ab2811 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2811 は 48 報の論文で使用されています。

  • Huber S  et al. Recruitment of Host Nuclear Pore Components to the Vicinity of Theileria Schizonts. mSphere 5:N/A (2020). PubMed: 32024710
  • Hazawa M  et al. Karyopherin-ß1 Regulates Radioresistance and Radiation-Increased Programmed Death-Ligand 1 Expression in Human Head and Neck Squamous Cell Carcinoma Cell Lines. Cancers (Basel) 12:N/A (2020). PubMed: 32276424
  • Oostdyk LT  et al. An epilepsy-associated mutation in the nuclear import receptor KPNA7 reduces nuclear localization signal binding. Sci Rep 10:4844 (2020). PubMed: 32179771
  • Mäntylä E  et al. Cytoplasmic Parvovirus Capsids Recruit Importin Beta for Nuclear Delivery. J Virol 94:N/A (2020). PubMed: 31748386
  • Yokoi A  et al. Mechanisms of nuclear content loading to exosomes. Sci Adv 5:eaax8849 (2019). PubMed: 31799396
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 22 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Dr. Andrew Bowman

Verified customer

投稿 Apr 10 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3 cells)
Permeabilization
No
Specification
NIH-3T3 cells
Blocking step
1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Nov 02 2015

Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Syrian hamster Cell lysate - whole cell (BHK)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
BHK
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 28 2013

Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
MDCK
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 28 2013

Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Cell lysate - whole cell (MDBK)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
MDBK
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 28 2013

Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (COS cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
75 µg
Specification
COS cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 17 2013

Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (NRK cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
NRK cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 17 2013

Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293F and HeLa cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
75 µg
Specification
HEK-293F and HeLa cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 17 2013

Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung,Kidney & Spleen extracts)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
100 µg
Specification
Lung,Kidney & Spleen extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 17 2013

Western blot abreview for Anti-KPNB1 antibody [3E9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK cells)
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Loading amount
100 µg
Specification
MDCK cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 May 14 2013

1-10 of 22 Abreviews or Q&A

  •  戻る
  • 1
  • 2
  • 3
  • 次へ 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

技術情報や製品情報をメールで受け取る 登録
製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
  • ステムセル
製品・タイプ別
  • 一次抗体
  • 二次抗体
  • バイオケミカルズ
  • アイソタイプ・コントロール
  • マルチカラー・セレクター
  • キット
  • ローディング・コントロール
  • ライゼート
  • ペプチド
  • タンパク質
  • スライド
  • タグ抗体・細胞マーカー
  • その他試薬
サービス&サポート
  • サポート
  • よくあるお問い合わせ
  • プロトコール&トラブルシュート
  • ご購入に関して
  • お問い合わせフォーム
  • トレーニング
  • ターゲット別に見る
アブカムについて
  • 企業ウェブサイト
  • 投資家の皆様へ
  • 企業情報
  • 採用情報
  • アブカムについて
イベント
  • 展示会
  • カンファレンス
ブログ

ブログを読む

International websites
  • abcam.com
  • abcam.cn

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
© 1998-2021 Abcam plc. All rights reserved.