KN-93 (water soluble), CaMK II inhibitor (ab120980)
Key features and details
- Potent, specific CaMK II inhibitor
- CAS Number: 1188890-41-6
- Purity: > 99%
- Soluble in water to 100 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
KN-93 (water soluble), CaMK II inhibitor -
製品の詳細
Potent, specific CaMK II inhibitor -
精製度
> 99% -
CAS 番号
1188890-41-6 -
構造式
製品の特性
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体系名
N-[2-[[[(2E)-3-(4-Chlorophenyl)-2-propen-1-yl]methylamino]methyl]phenyl]-N-(2-hydroxyethyl)-4-methoxybenzenesulfonamide phosphate -
分子量
599.03 -
分子式
C26H29ClN2O4S.H3PO4 -
PubChem 登録番号
16760530 -
保存方法
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. -
溶解性
Soluble in water to 100 mM and in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
Clc3ccc(/C=C/CN(C)Cc2ccccc2N(CCO)S(=O)(=O)c1ccc(OC)cc1)cc3.O=P(O)(O)O -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120980の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120980, KN-93 (water soluble), CaMK II inhibitor
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ab40754 staining cyclin D1 in MCF7 cells treated with KN-93 (water soluble) (ab120980), by ICC/IF. Decrease in cyclin D1 expression correlates with increased concentration of KN-93 (water soluble), as described in literature.
The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab120980 (KN-93 (water soluble)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab40754 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (3)
ab120980 は 3 報の論文で使用されています。
- Yong XLH et al. Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIa phosphorylation of the GluN2A subunit. Cell Rep 36:109338 (2021). PubMed: 34233182
- Kaiser TS et al. The genomic basis of circadian and circalunar timing adaptations in a midge. Nature 540:69-73 (2016). PubMed: 27871090
- Teo CS & Chu JJ Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein. J Virol 88:1897-913 (2014). PubMed: 24284321