Anti-KMT6 / EZH2 抗体 - ChIP Grade (ab195409)
Key features and details
- Rabbit polyclonal to KMT6 / EZH2 - ChIP Grade
- Suitable for: ChIP, WB, ChIP-sequencing
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-KMT6 / EZH2 antibody - ChIP Grade
KMT6 / EZH2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KMT6 / EZH2 - ChIP Grade -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, WB, ChIP-sequencingmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cynomolgus monkey -
免疫原
Recombinant fragment corresponding to Mouse KMT6/ EZH2 aa 1-350.
Database link: Q61188 -
ポジティブ・コントロール
- Chromatin prepared from K562 cells; HeLa nuclear extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab195409の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP |
Use at an assay dependent concentration.
Use 2 μg/ChIP for chromatin prepared from 4x106 cells. |
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WB |
1/1000. Predicted molecular weight: 85 kDa.
|
|
ChIP-sequencing |
Use at an assay dependent concentration.
Use 2 μg/ChIP for chromatin prepared from 4x106 cells |
特記事項 |
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ChIP
Use at an assay dependent concentration. Use 2 μg/ChIP for chromatin prepared from 4x106 cells. |
WB
1/1000. Predicted molecular weight: 85 kDa. |
ChIP-sequencing
Use at an assay dependent concentration. Use 2 μg/ChIP for chromatin prepared from 4x106 cells |
ターゲット情報
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機能
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. -
組織特異性
Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. -
配列類似性
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain. -
発生段階
Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary. -
翻訳後修飾
Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 102146280 Cynomolgus monkey
- Entrez Gene: 2146 Human
- Entrez Gene: 14056 Mouse
- Omim: 601573 Human
- SwissProt: Q4R381 Cynomolgus monkey
- SwissProt: Q15910 Human
- SwissProt: Q61188 Mouse
- Unigene: 444082 Human
see all -
別名
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
see all
画像
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ChIP results obtained with ab195409 directed against KMT6 / EZH2.
ChIP assays were performed using K562 cells, ab195409 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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ChIPseq results obtained with ab195409 directed against KMT6 / EZH2.
ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of ab195409. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure A and B, respectively), and in two genomic regions containing the MYT1 gene on chromosome 20 and the HOX cluster on chromosome 7 (figure C and D).
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Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409) at 1/1000 dilution + HeLa nuclear extract at 40 µg
Predicted band size: 85 kDaThe antiody was diluted in TBS-Tween containing 5% skimmed milk.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab195409 は 9 報の論文で使用されています。
- Xu W et al. Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation. Cancer Cell Int 20:116 (2020). PubMed: 32308561
- Cao W et al. Multi-faceted epigenetic dysregulation of gene expression promotes esophageal squamous cell carcinoma. Nat Commun 11:3675 (2020). PubMed: 32699215
- Liu J et al. Long noncoding RNA LINC01578 drives colon cancer metastasis through a positive feedback loop with the NF-?B/YY1 axis. Mol Oncol 14:3211-3233 (2020). PubMed: 33040438
- Cheng W et al. LncRNA MAGI2-AS3 Overexpression Sensitizes Esophageal Cancer Cells to Irradiation Through Down-Regulation of HOXB7 via EZH2. Front Cell Dev Biol 8:552822 (2020). PubMed: 33330444
- Wu D et al. EZH2 promotes the expression of LPA1 by mediating microRNA-139 promoter methylation to accelerate the development of ovarian cancer. Cancer Cell Int 20:551 (2020). PubMed: 33292225
- He W et al. Downregulation of long noncoding RNA FENDRR predicts poor prognosis in renal cell carcinoma. Oncol Lett 17:103-112 (2019). PubMed: 30655744
- Qiu Z et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
- Garland W et al. A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. Cell Rep 29:1800-1811.e6 (2019). PubMed: 31722198
- Rotolo A et al. Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting. Cancer Cell 34:596-610.e11 (2018). PubMed: 30300581