Anti-KMT2B / MLL4 抗体 (ab104444)
Key features and details
- Goat polyclonal to KMT2B / MLL4
- Suitable for: ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KMT2B / MLL4 antibody
KMT2B / MLL4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to KMT2B / MLL4 -
由来種
Goat -
アプリケーション
適用あり: ELISA, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig, Monkey, Zebrafish, Gorilla, Common marmoset -
免疫原
Synthetic peptide corresponding to Human KMT2B/ MLL4 aa 2703-2715 (C terminal). Entrez gene ID: 9757
Sequence:CNCGAKRCRRFLN
Database link: Q9UMN6 -
ポジティブ・コントロール
- IHC-P: Human brain cortex and testis tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Immunoaffinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104444の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation. -
組織特異性
Widely expressed. Highest levels in testis. Also found in brain, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas. -
配列類似性
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.
Contains 3 A.T hook DNA-binding domains.
Contains 1 CXXC-type zinc finger.
Contains 1 FYR C-terminal domain.
Contains 1 FYR N-terminal domain.
Contains 3 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 1 SET domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9757 Human
- Entrez Gene: 75410 Mouse
- Entrez Gene: 102550344 Rat
- Omim: 606834 Human
- SwissProt: Q9UMN6 Human
- SwissProt: O08550 Mouse
- Unigene: 676457 Human
- Unigene: 92236 Human
see all -
別名
- Histone-lysine N-methyltransferase 2B antibody
- HRX2 antibody
- KIAA0304 gene product antibody
see all
画像
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ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human brain cortex tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.
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ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human testis tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab104444 は 3 報の論文で使用されています。
- Swaminathan J et al. Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis. Front Oncol 12:855167 (2022). PubMed: 35600406
- Su CH et al. Regulation of IL-20 Expression by Estradiol through KMT2B-Mediated Epigenetic Modification. PLoS One 11:e0166090 (2016). ChIP, IHC-P ; Human . PubMed: 27806114
- Fulcoli FG et al. Rebalancing gene haploinsufficiency in vivo by targeting chromatin. Nat Commun 7:11688 (2016). PubMed: 27256596