Anti-KLF15 抗体 (ab2647)

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Western blot - Anti-KLF15 antibody (ab2647)

    Key features and details

    • Goat polyclonal to KLF15
    • Suitable for: IP, WB, IHC-P
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-KLF15 antibody
      KLF15 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to KLF15

    • 由来種

      Goat

    • アプリケーション

      適用あり: IP, WB, IHC-Pmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Pig

    • 免疫原

      Synthetic peptide within Human KLF15 aa 1-100 (N terminal) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)

       

      We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab2647の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IP
    Use at an assay dependent concentration. PubMed: 24407292
    WB
    Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).

    A 1 hour primary incubation is recommended for this product. Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31).
    IHC-P
    Use at an assay dependent concentration. PubMed: 26378916
    特記事項
    IP
    Use at an assay dependent concentration. PubMed: 24407292
    WB
    Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).

    A 1 hour primary incubation is recommended for this product. Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31).
    IHC-P
    Use at an assay dependent concentration. PubMed: 26378916

    ターゲット情報

    画像

    • Western blot - Anti-KLF15 antibody (ab2647)
      Western blot - Anti-KLF15 antibody (ab2647)
      Lane 1 : Anti-KLF15 antibody (ab2647) at 1 µg/ml
      Lane 2 : Anti-KLF15 antibody (ab2647) at 0.5 µg/ml

      Lane 1 : HepG2 nuclear lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 44 kDa

    参考文献 (18)

    ab2647 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2647 は 18 報の論文で使用されています。

    • Puttipanyalears C  et al. Quantitative STAU2 measurement in lymphocytes for breast cancer risk assessment. Sci Rep 11:915 (2021). PubMed: 33441653
    • Mao L  et al. Histone Deacetylase 11 Contributes to Renal Fibrosis by Repressing KLF15 Transcription. Front Cell Dev Biol 8:235 (2020). PubMed: 32363192
    • El-Mayet FS  et al. Two Pioneer Transcription Factors, Krüppel-Like Transcription Factor 4 and Glucocorticoid Receptor, Cooperatively Transactivate the Bovine Herpesvirus 1 ICP0 Early Promoter and Stimulate Productive Infection. J Virol 94:N/A (2020). PubMed: 31776270
    • Ostler JB  et al. The Glucocorticoid Receptor (GR) Stimulates Herpes Simplex Virus 1 Productive Infection, in Part Because the Infected Cell Protein 0 (ICP0) Promoter Is Cooperatively Transactivated by the GR and Krüppel-Like Transcription Factor 15. J Virol 93:N/A (2019). PubMed: 30602606
    • Hirata Y  et al. Hyperglycemia induces skeletal muscle atrophy via a WWP1/KLF15 axis. JCI Insight 4:N/A (2019). PubMed: 30830866
    View all Publications for this product

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