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The Multiplex Circulating miRNA Assay allows miRNA profiling direct from crude biofluids, including plasma, serum and exosomes, without the need for RNA purification. Find out how miRNA profiling direct from biofluids can benefit your experiment:
Reduced variability. Remove the unreliable RNA purification step in your sample preparation and get more consistent data.
Simplified workflow. Take less time from sample to data. RNA extraction adds significantly to the steps required to profile miRNAs across multiple samples. The Multiplex Circulating miRNA Assay with crude biofluids simplifies the workflow and cuts down assay time.
Smaller sample input. Profile miRNAs direct from as little as 10–40 μl crude biofluids. With the low abundance of miRNAs in biofluids, miRNA analysis typically requires a large quantity of biofluid for RNA extraction. Cut out this step to save your sample.
In this technical note, we demonstrate how this assay can be used for miRNA profiling with PCR sensitivity directly from as little as 20 µL of serum or plasma with no need for RNA purification, or from less than 100 pg of purified RNA. With our easy to use bioinformatics tools, we demonstrate that consistent miRNA profiles are obtained from plasma obtained using a variety of blood collection methods, including heparin.
miRNA profiling in crude digests versus extracted DNA. There was a close correlation between miRNA expression profiles obtained from purified miRNA and those obtained from crude serum.